IBM SPSS Data Collection Technical Support Mastery v1 :: P2020-012 Dumps Questions and answers | braindumps | Online Test Prep

IBM SPSS Data Collection Technical Support Mastery v1













IBM SPSS Data Collection Technical Support Mastery v1
P2020-012 Dumps Questions | Online Test Prep | P2020-012 Braindumps


We provide P2020-012 dumps taken from real P2020-012 exam with 100% money back guarantee



IBM P2020-012 Dumps and Practice Tests with Real Question
Collecting P2020-012 dumps from many sources is not bad, but they suggest to rely on just good service. Killexams.com provide latest, valid and updated P2020-012 real exam questions that most of websites do not have. Their team keep contacted with test takers and their special resources to get updated and latest P2020-012 braindumps.

000-286 | 000-N52 | LOT-915 | 000-M91 | C2090-461 | C2180-273 | 000-S32 | 000-M234 | 000-N05 | C2140-820 | 000-037 | A2040-918 | P2170-015 | C2070-586 | 000-SS1 | 000-536 | 000-734 | C2010-658 | 000-530 | M2090-234 |



Can someone unravel my doubt on automatic linear modelling supplied by using IBM-SPSS?

2 years in the past

Birmingham city school

dear Duraisamy,

I have under no circumstances used this automatic variable merging characteristic in SPSS and i individually would no longer suggest it. in case you do you utilize it you are going to need to justify the choices SPSS has made for you in merging your variables.

I additionally noticed that the computerized regression model you selected is Forwards stepwise. i might advocate backwards stepwise, according to the assistance given with the aid of Andy container in his ebook Discovering records the use of SPSS. once again, if you use an automated system for adding or getting rid of variables be sure to explain and justify the selections it has made.

In abstract, each computerized points can be advantageous however should still most effective be employed if you can explain what they're doing and why the ultimate model acquired is in some experience the superior (e.g. according to the precept of parsimony).


TGF-β induces miR-100 and miR-125b but blocks let-7a through LIN28B controlling PDAC development

cell lifestyle

BxPC-3, PANC-1, and COLO357 cells were got from American type way of life assortment. S2-007 and S2-028 cells were received from Prof. Thomas Gress (institution medical institution Marburg, Marburg, Germany). PANC-1 stably expressing TGF-β1 or empty vector have been acquired from Prof Matthias Löhr and Dr Rainer Heuchel (Karolinska institution hospital, Stockholm, Sweden). CHX45 cells were received from Dr Bruno Sainz Jr (branch of Biochemistry, Universidad Autónoma de Madrid, Madrid, Spain)33. BxPC-3, COLO357, CHX45 and both fundamental telephone cultures from PDAC sufferers (LPC006 and LPC167)forty five were grown in RPMI-1640 medium supplemented with 10% fetal calf serum, 2 mM L-glutamine, one hundred U ml−1 penicillin, and a hundred mg ml−1 streptomycin. PANC-1, S2-007, and S2-028 have been maintained in Dulbecco’s modified Eagle medium supplemented with 10% fetal calf serum, 2 mM L-glutamine, 100 U ml−1 penicillin, and one hundred mg ml−1 streptomycin. PANC-1 and S2-007 good miRZip traces had been extended in puromycin (Gibco). All cells strains were tested month-to-month for mycoplasma illness (MycoAlert, Lonza).

Transfections and cellphone treatments

Mimics, inhibitors, and Silencer choose siRNAs had been purchased from Ambion. Mimics and inhibitors have been transfected into cells using HiPerFect Transfection Reagent (Qiagen, Crawley, UK) even as Silencer opt for siRNAs were transfected the use of Lipofectamine RNAiMAX (Invitrogen) following the company’s advised protocol. until otherwise detailed 5 nM of mimics, 100 nM of anti-miRs or forty nM of siRNAs were transfected for forty eight h. For experiments of siRNA co-transfection, 20 nM of every siRNA was used. Mimics: poor manage #1 mimic (AM17110), pre-miR-one hundred (PM10188), pre-miR-125b (PM10148). Inhibitors: anti-miR bad control #1 (AM17010), anti-miR-a hundred (AM10188), anti-miR-125b (AM10148). siRNAs: bad control #2 (4390846), siLIN28B (4392420 s52479), siSMAD2 (4392420 s8397), siSMAD3 (4392420 s8400). For experiments of miRNA overexpression for 12 days, cells have been transfected with the miRNA mimics (5 nM) and each 3 days cells have been cut up and re-transfected with additional miRNAs mimics. For TGF-β cures, PANC-1 cells were handled with 5 nM TGF-β (R&D systems) for the indicated time. SB431542 (Sigma) turned into used at 2.5 μg ml−1 in mixture with TGF-β for twenty-four h. Gemcitabine (GEM) was used at 1 µM for twenty-four h.

technology of miR-Zip stable strains

PANC-1 and S2-007 miR-a hundred and miR-125b knockdown stable lines have been generated using miRZipTM lentivector-based mostly anti-microRNAs know-how (device Biosciences), following the brand’s protocol. miRZip lentiviral vectors stably inhibit the miRNA of hobby by way of expressing a single-stranded shRNA which is recognized by DICER and processed to generate functional anti-miRNAs for the goal miRNA. miRZip100 (Cat# MZIP100-PA-1), miRZip125b (Cat# MZIP125b-PA-1) or miRZip control (Cat# MZIP000-PA-1) were packaged the use of the pPACKH1 lentivector packaging equipment (LV500A-1, SBI) in 293TN produced line (LV900A-1, SBI) and transduced into PANC-1 or S2-007 cells. Cells were selected the use of puromycin (1.6 µg ml−1) for 7 days. The chosen pool turned into then subjected to FACS sorting for GFP expression and 1 cellphone per neatly changed into seeded in a ninety six smartly plate. Clones were left to develop and as a result screened using the Cells-to-Cts protocol followed via QuantiMir RT kit (RA420A-1, SBI) the use of custom designed probes for Zip-one hundred and Zip-125b. The clones with the optimum Zip-one hundred or Zip-125b expression have been chosen for phenotypic experiments. For in vivo metastasis assay S2-007 clones were transduced with lentiviral particles carrying red-shifted Luciola italica luciferase transgene (RediFect crimson-Fluc-Puromycin, PerkinElmer). To check the impact of TGF-β in vivo when miR-one hundred or miR-125b are inhibited, PANC-1 miR-one hundred Zip and miR-125b Zip clones had been stably transduced with a lentiviral vector carrying TGFB-1 ORF (Cat# EX-Z5895-Lv151, GeneCopoeiaTM) or vector handle (Cat# EX-NEG-Lv151, GeneCopoeiaTM). Cells had been selected using G418 (800 µg ml−1) for 7 days.

CRISPR-Cas9-mediated KO traces

All sgRNAs used for CRISPR-mediated PANC-1 KO strains were designed using the CRISPR design tool (https://killexams.com/vendors-exam-list). To generate mir-one hundred and mir-125b KO clones, pairs of sgRNAs were chosen within the miRNA genomic locus (see Fig. 4a) with the aim of disrupting part or the complete miRNA locus. For deletion of MIR100HG promoter region (MIR100HG∆P), pairs of sgRNAs had been chosen to eradicate the SMAD2/3 peaks envisioned via MACS2 from their ChIP-seq experiments. at last, to generate LIN28B KO clones a single sgRNA concentrated on the genomic region downstream of the AUG translation beginning website codon changed into used. Oligonucleotides containing sgRNA sequences had been cloned into lentiCRISPRv2 vector (a present from Feng Zhang; Addgene #5296146) following the lentiCRISPRv2 and lentiGuide oligo cloning protocol deposited by using the Zhang lab on Addgene. Oligonucleotides sequences are supplied in desk 1. For LIN28B, miR-a hundred and miR-125b KO clones, lentiCRISPRv2 containing the goal sgRNA sequences have been transfected in PANC-1 cells using Lipofectamine LTX (Invitrogen). At 24 h submit-transfection, medium was changed and cells have been chosen with 10 μg mL−1 of puromycin (Gibco). After forty eight h of antibiotic alternative cells have been left to develop and validation of CRISPR-Cas9 genome modifying become assessed by using Sanger sequencing of the PCR items of activity. Primers used for PCR amplification of genomic loci edited with the aid of CRISPR-Cas9 are indicated in desk 2. Cells with the desire genome modifying were FACS sorted into 96 well plates as single cells and clones have been left to develop for 7 days. chosen clones have been further validated by Sanger sequencing and KO of the gene of pastime was confirmed with the aid of RT-qPCR in case of miR-a hundred and miR-125b or western blot for LIN28B. The MIR100HG∆P line was generated by lentiviral an infection in PANC-1 cells. briefly, lentiCRISPRv2 containing the goal sgRNA sequences were co-transfected with packaging plasmids pMD2.G and psPAX2 (each presents from Didier Trono; Addgene plasmids #12259 and #12260) in HEK293T cells the usage of Lipofectamine LTX (Invitrogen). Medium was refreshed after 8 h and lentivirus-containing medium become gathered 60 h after transfection. PANC-1 cells have been infected with lentivirus supernatant supplemented with 8 μg ml−1 polybrene (Sigma). At 24 h put up-an infection medium become refreshed and cells had been selected with 10 μg mL−1 of puromycin for 48 h. Cells had been left to grow and deletion of the promoter area become proven via Sanger sequencing.

desk 1 Oligonucleotides for sgRNA cloning table 2 PCR primers used in this look at RNA isolation and RT-qPCR assays

total RNA from cultured cells became extracted the use of TRI Reagent (Sigma) following the company’s directions together with DNase I remedy. qRT-PCR of mature miRNAs turned into carried out using TaqMan Small RNA Assays (applied Biosystems) with assays for hsa-miR-a hundred (000437), hsa-miR-125b (000449), hsa-let-7a (000377), has-miR-200a (000502). Small nuclear RNA U44 (001094), U47 (001223), or U6 (001973) have been used as endogenous controls. For gene expression, cDNA changed into synthesized from 1 μg of purified DNase-handled RNA using RevertAidTM M-MuLV reverse transcriptase and random hexamer primers (Thermo Scientific), in keeping with the manufacturer’s protocols. They performed qRT-PCR on a StepOne™ actual-Time PCR system the usage of quickly SYBR® green master combine (both from applied Biosystems). The primer sequences used are stated in desk 2.

Sphere formation assay

BxPC-three, PANC-1, PANC-1 ZiP strong strains, PANC-1 CRISPR-Cas9 KO strains or PANC-1 TGF-β stables had been plated as single mobile suspension in 6 well ultra-low attachment plates (Corning) at a density of two × 103 cells/well. Cells were grown in serum free DMEM/F12 medium (Gibco) supplemented with B27 (1:50, Gibco) and 20 ng ml−1 simple fibroblast grown aspect (bFGF, Biolegend). Tumor spheres had been counted at day 7 when customarily reached a measurement of >seventy five μm. Sphere formation effectivity (SFE) was calculated because the variety of spheres fashioned at day 7 divided via the variety of cells seeded and expressed as a percent.

Western blotting

whole cellphone lysates were prepared in RIPA buffer (Sigma) and quantified the use of Bradford Protein Assay (Bio-Rad). Twenty micrograms of lysates have been resolved on Bolt® four–12% Bis-Tris Plus gels the use of BoltTM MOPS SDS operating buffer and transferred to a Hybond C tremendous nitrocellulose membrane (GE Healthcare). After blocking off to keep away from non-selected binding in 5% milk in PBST for 1 h at room temperature, membranes had been incubated with the specific basic antibodies overnight at 4 °C. the following primary antibodies have been used: E-cadherin (Takara Bio Inc., M106, clone HECD-1, 1:one thousand dilution), Occludin (cell Signaling, #5446, 1:1000 dilution), Vimentin (phone Signaling, #5741, 1:3000 dilution), ZEB1 (Santa Cruz, sc-25388, 1:500 dilution), SNAI1 (Santa Cruz, sc-28199, 1:500 dilution), LIN28B (phone Signaling, #4196, 1:one thousand dilution), LIN28A (telephone Signaling, #3978, 1:1000 dilution), SMAD2/3 (telephone Signaling, #8685, 1:a thousand dilution), β-actin (Abcam, ab8227, 1:200,000 dilution), GAPDH (Santa Cruz, sc-137179, 1:10,000 dilution). Following incubation with the selected HRP-conjugated antibody (Dako, #P0447 or #P0448, 1:2,500 dilution), chemiluminescence sign become detected the use of AmershamTM ECLTM Western blotting detection reagents (GE Healthcare) and Amersham HyperfilmTM ECL (GE Healthcare). Uncropped scans of the most vital blots are shown in Supplementary Fig. 13.

Immunofluorescence staining

Cells grown on glass coverslips have been fixed in four% paraformaldehyde for 10 min at room temperature. Cells had been washed twice with PBS (15 min each) and permeabilised with 0.3% (v/v) Triton X-a hundred for 10 min. blockading buffer (1% w/v BSA, 2% v/v FCS, 5% v/v goat serum in PBS) turned into introduced for 30 min and cells had been then stained with anti-E-cadherin antibody (Takara Bio Inc., M106, clone HECD-1, 1:1000 dilution) and Alexa Fluor® 555 (Invitrogen, A-21422, 1:500 dilution). F-actin became detected with phalloidin-Alexa Fluor 488® (Invitrogen, A12379, 1:500 dilution) and nuclei were visualized using TO-pro®-three (Invitrogen, T3605, 1:a thousand dilution). images were acquired with a Zeiss LSM 510 META confocal microscope (Carl Zeiss Ltd).

Wound-curative migration assays

Confluent cells were scratch-wounded using a 20 μl pipette tip and mobile particles have been removed via washing with PBS. section-contrast images have been taken at the indicated time features at certain wound sites the usage of EVOS microscope with a 10 × aim. For time-lapse experiments, cellphone migration changed into assessed by way of phase-distinction videomicroscopy with sequences taken every 30 min for twenty-four h after scratching using Axiovert a hundred MetaMorph Microscope (Carl Zeiss Ltd) geared up with a humidified 37 °C incubation chamber. For wound-curative assay in adenocarcinoma primary traces LPC006 and LPC067, cells have been transfected with one hundred nM of anti-miR-NC, anti-miR-100 or anti-miR-125b and seeded in ninety six neatly plates (30 × 104 cells/smartly), the place artificial wounds have been made the use of a pipette tip. The means of the cells emigrate changed into evaluated by way of comparing the pixels of the wound tracks in the images taken at first of the publicity (time 0) with these taken 2 hourly for 8 h and then finally at 16 h. Migration become assessed using the LeicaDMI300B (Leica) migration station integrated with the Scratch-Assay 6.1 utility (Digital-mobile Imaging Labs, Keerbergen, Belgium).

circulate cytometry

To identify CD133 nice inhabitants, 1 × 106 S2-007 Zip cells have been trypsinized, washed with PBS and stained with antiCD133/1-APC (Miltenyi Biotec) or applicable isotype-matched manage antibody for 30 min at four °C. The labelled cells had been washed in PBS and subjected to circulation cytometry on a FACS Canto II (BD Bioscieces). data were analyzed with FlowJo v8.8.9 utility.

Apoptosis assay

LPC006 and LPC067 cells were transfected with one hundred nM of anti-miR-NC, anti-miR-100, or anti-miR-125b and day after today were treated with 1 µM gemcitabine (GEM) chemotherapy for twenty-four h. Following treatments, cells were washed twice with PBS and glued in 4% PFA for 15 min. Cells were then resuspended in a solution containing eight μg ml−1 bisbenzimide HCl and incubated for 15 min. Cells were noticed on glass slides and have been examined with the aid of fluorescence microscopy (Leica, Wetzlar, Germany). a total of 200 cells from randomly chosen microscopic fields have been counted, and the percentage of cells displaying chromatin condensation and nuclear fragmentation relative to the entire number of counted cells (apoptotic index) turned into calculated. The average percentage of apoptosis precipitated with the aid of GEM and anti-miR-NC (negative handle) changed into 19% and 12% in the LPC006 and LPC067 cells, respectively. These values have been set as a hundred%, with the intention to exhibit the change after treating with the different anti-miRs more evidently. Apoptosis induction <20% shows that both mobilephone traces don't seem to be very delicate to GEM.

Luciferase reporter assays

PANC-1 cells were seeded onto 24 well plates at a density of fifty × 104 cells/neatly in antibiotic-free medium. Twenty-4 hours later, cells were co-transfected with pre-miR-100 or pre-miR-125b or negative manage (100 nM) at the side of the three’UTR of relevant genes reporter constructs (pLightSwitch_3UTR GoClone vectors, SwitchGear Genomics) at a hundred ng/neatly the use of Lipofectamine2000 (Invitrogen). After 24 h, cells had been washed with PBS and 50 μl of passive lysis buffer (Promega) changed into delivered to every neatly. After a 20-min incubation lysates were transferred to an optical nice ninety six smartly plate (OptiPlate, PerkinElmer) and luciferase undertaking become measured the use of the LightSwitch Assay equipment (SwitchGear Genomics) as directed by using the company. The resulted light emission changed into examine the use of the countless M200 plate reader (Tecan) and the suggest luciferase undertaking for each and every precursor miRNA is shown relative to the mean for the terrible control.

miRNA nCounter profiling

The Nanostring nCounter Human miRNA Expression Array (https://killexams.com/questions-and-answers) was used to acquire miRNA expression profiles47. nCounter miRNA pattern preparation became carried out according to the brand’s directions (NanoString technologies). Differential expression analysis between diverse telephone traces and PANC-1 cells treated with TGF-β or automobile become performed in R, the usage of nCounter uncooked values as input for DESeq2 analysis (https://killexams.com/exam-simulator).

AGO2 RNA immunoprecipitation

PANC-1 cells were seeded in 15 cm dishes (5 × 106 cells/dish; three dishes had been used for each and every antibody proven) and transfected with 0.5 nM of pre-NC, pre-miR-one hundred and pre-miR-125b for twenty-four h. AGO2-RIP was performed as up to now described through their group48. briefly, following transfection cells had been washed in cold PBS, scraped in PBS and accrued by way of centrifugation. Pellets have been then resuspended in lysis buffer (20 mM Tris-HCl pH7.5, 150 mM KCl, 0.5% NP40, 2 mM EDTA, 1 mM NaF, 0.5 mM DTT, one hundred sixty U ml−1 RNAsin and protease and phosphates inhibitors) and pre-cleared with Protein G sepharose beads (Sigma) for 2 h at 4°C. part of cleared lysates (10%) turned into used as enter and the remainder were incubated with Protein G sepharose beads conjugated with anti-AGO2 (11A9, SAB4200085, Sigma-Aldrich) or anti-IgG (Sigma-Aldrich) for four h at four°C. After washing, 10 μl of the immunoprecipitate turned into kept for western blot evaluation and the the rest was handled with DNAse I and proteinase ok for 20 min at room temperature. RNA turned into extracted using phenol/ chloroform and and ethanol/sodium acetate precipitation. RNA changed into then quantified the usage of Nanodrop.

Chromatin immunoprecipitation

PANC-1 cells had been treated with 5 nM TGF-β for twenty-four h and chromatin immunoprecipitation (ChIP) become carried out as follows49: in short cells had been crosslinked with 1% formaldehyde, chromatin became organized and incubated with 10 µg of SMAD2/3 antibody (R&D, AF3797) and one hundred µl of Dynabeads Protein A (10002D; Invitrogen) overnight at four°C. The immune-precipitated complex became washed with RIPA buffer and TE buffer, adopted through de-crosslinking for sixteen h. DNA turned into then treated with RNase and Proteinase okay at 1 mg ml−1 and purified with phenol-chloroform and sodium chloride precipitation. For ChIP-PCR the primer sequences are as observe: SNAI1 (ahead: 5’-CGCTCCGTAAACACTGGAT-three’; Reverse: 5’-GAAGCGAGGAAAGGGACAC-three’), MIR100HG (forward: 5’-AGCAAACACATTTCAGGCAGT-3’; Reverse: 5’-GGCTACCTGACTGATGAGTG-three’), HBB (forward: 5’-GCTTCTGACACAACTGTGTTCAC-three’; Reverse: 5’-CACCAACTTCATCCACGTTCACC-three’).

ChIP-seq library practise and bioinformatics evaluation

ChIP-seq libraries have been constructed using NEBNext extremely II DNA library prep equipment for Illumina equipment (NEB), in keeping with the manufacturer’s protocols. Ten nanograms of DNA turned into used for library preparation. Single-end reads of fifty nt in length have been generated using a HiSeq 2000 instrument (Illumina). Sequences have been aligned to the human reference genome (meeting hg19, February 2009) the use of Bowtie 1.0. and height calling became performed the usage of MACS v1.four the use of default settings. ChIP-seq statistics of CTCF, H3K4me3, and H3K27ac from PANC-1 or Pancreata have been received from ENCODE and plotted using IGV genome browser.

hi-C analyses

hello-C information of PANC-1 comes from ENCODE. Analyses and graphs had been performed the usage of online equipment from https://killexams.com/search.

RNA-seq and AGO2-RIP-seq library instruction

RNA libraries for RNA-seq and AGO2-RIP-seq had been organized with TruSeq RNA Library Prep equipment v2 (Illumina), according to the brand’s protocols. Paired-conclusion sequences (reads) of one hundred nt in size had been then generated the usage of a HiSeq 2000 instrument (Illumina).

Processing of RNA-seq and AGO2-RIP-seq facts

The best of the reads contained in the fastq information obtained on the end of the sequencing become assessed the usage of FastQC edition 0.10.1 (https://killexams.com/it-vendor-list). The reads from the fastq info, for each pattern, were then mapped on the reference human genome, version hg19, received from the institution of California Santa Cruz (united states) genome browser (https://killexams.com/project-management states of america.edu/) by using TopHat. For isoform level analysis (miRNA goal identification) RPKM normalized values have been produced with Partek Genomic go well with application (Partek Inc) the usage of the bam data attained after the TopHat runs, as input. For gene level analysis (TGF-β medicine) raw counts were produced the usage of htseq edition 0.6.1 (https://killexams.com/exam-list/Medical) with human RefSeq annotation and used for differential expression evaluation with DESeq2 from the Bioconductor (https://killexams.com/language-exams).

RIP adopted by using unbiased Sequence Enrichment (RIP-USE)

We developed RIP-USE for miRNA-target identification in an effort to identify canonical and noncanonical objectives for miR-one hundred and miR-125b. It integrates AGO2-RIP-seq with RNA-seq and independent motif enrichment analysis to establish enriched motifs complementary to any a part of the miRNAs enriched in the transcripts loaded onto AGO2 upon expression of miR-125b or miR-one hundred in telephone traces. The characteristic of those motifs in regulating ambitions via miRNA interplay became then established by performing cumulative distribution analyses evaluating the world expression of transcripts containing recognized websites versus transcripts devoid of them, upon miRNA expression. It carries diverse steps (Fig. 6a): (1) miRNA overexpression in phone lines, (2) AGO2-RIP-seq of the cells overexpressing the miRNA of interest or a poor manage (n.c.), (3) RNA-seq of the cells overexpressing the miRNA of activity or a terrible manage (n.c.). After mapping of the sequencing reads followed by gene expression evaluation (4) the transcripts are then sorted from the most enriched to the least enriched in AGO2 for AGO2-RIP-seq, in addition to from the least down-regulated to most up-regulated for RNA-seq. since that continually the area of the miRNAs that base pairs with their targets correspond to a 6–8 mer found in the 5’ half known as the ‘seed’50 the genes enriched for AGO2 and the ones down-regulated after the expression of the miRNA of hobby should still be enriched of phrases 6–8 bases lengthy complementary (canonical pairing) or partly complementary (noncanonical pairing) with the seed of the overexpressed miRNAs. on the grounds that this principle, (5) to locate bona fide pursuits of the overexpressed miRNAs they used equipment that unbiasedly retrieve enriched words 6–eight bases lengthy inside chosen regions of sorted transcripts38,forty,fifty one for both AGO2-RIP and RNA-seq. They evaluated even if (6) words representing noncanonical interaction derived from regions of enriched transcripts onto AGO2 for RIP-seq overlap with the ones from regions of down-regulated transcripts for RNA-seq. finally (7) they validated whether the transcripts containing these 6–8mers are definitely regulated with the aid of the miRNAs, evaluating no matter if transcripts containing these words were tremendously down-regulated compared to transcripts without those phrases in RNA-seq upon the overexpression of the miRNAs, the use of cumulative distribution evaluation (Fig. 6b,c).

Pathway analyses

Pathway analyses had been carried out the use of IPA (Qiagen, https://killexams.com/entry-test-preparation). Genes from the overlap between essentially the most enriched transcripts in AGO2-RIP-seq (n = 4000) and the excellent down-regulated transcripts in RNA-seq (1300) had been used as gene units for the evaluation (Fig. 7a,b). Expression values (Log2FC) have been also together with to gain the IPA Z-ranking that infers onto activation (high-quality Z rating) or inhibition (negative Z score) of the big enriched pathways. Networks of precise enriched IPA pathways had been constructed the usage of Cytoscape utility v3.four (https://killexams.com/exam-list/Banking). For IPA comparison evaluation, genes that have been up and down-regulated (Z-ranking > 1.5 and Z-rating < −1.5) have been analyzed.

analysis of mRNA and miRNA expression profiling from The melanoma Genome Atlas (TCGA) database

level 3 records of miRNA and mRNA expression profiling statistics from pancreatic adenocarcinoma samples (PPAD-TCGA) had been downloaded from the TCGA database (https://killexams.com/exam-list/Financial) and used to develop gene and miRNA expression matrices. Pearson correlation analyses between miRNAs in addition to between miRNAs and mRNAs of pastime had been calculated and graphed the usage of R (). They considered low LIN28B degree samples to be those that categorical less than 0.5 RSMEs ().

In vivo tumorigenicity and metastasis assays

For tumorigenesis assays, serial dilutions of S2-007 Zip cells diluted in 50 μl PBS have been injected subcutaneously in each flanks of 4- to five-week-old feminine athymic nude mice (Charles River Laboratories). control cells had been inoculated to the appropriate flank and Zip100 or Zip125b cells to the left. Tumor increase changed into documented for three weeks. For TGF-β tumorigenesis assay, 2 × 106 PANC-1 Zip cells stably expressing TGF-β1 or empty vector diluted in 50 μl PBS were injected subcutaneously in each flanks of four- to 5-week-old female athymic nude mice (Charles River Laboratories). Tumor growth changed into documented for 5 weeks. For metastasis assay, 25 × 104 S2-007 Zip cells stably expressing luciferase resuspended in 50 μl PBS have been intrasplenically injected into 4- to five-week-ancient feminine athymic nude mice (Charles River Laboratories). Seven days after injection the spleen become removed to avid increase of the tumor on the injection site. Tumor dissemination turned into monitored twice a week for 3 weeks by bioluminescence imaging using the IVIS Spectrum Imaging gadget (Caliper existence Sciences). in brief, mice had been injected intraperitoneally with a hundred and fifty mg kg−1 of luciferin (Promega, E1605) diluted at 15 mg mL−1 in sterile PBS and imaged after ~15 min. The dwelling graphic software (Caliper existence Sciences) was used to quantify photon emission and areas of hobby (ROI) changed into used to calculate the overall photons/2d (photons flux) emitted. The variety of animals utilized in all experiments mirrored the anticipated magnitude of response deliberating the range followed in outdated experiments. Mice had been randomly allocated to each group. Investigators had been no longer blinded. Animal experimental approaches were performed beneath the united kingdom domestic office project License number PPL 70/8448.

Laser seize microdissection (LCM) of tissues

Samples of matched tissues (common pancreas, n = eight; PDAC, n = 20; lymph-node metastasis, n = 20) have been bought from FFPE blocks of surgical specimens after pancreaticoduodenectomy (all resectable Stage IIB) at Hammersmith sanatorium, UK. Cells had been selectively isolated with the PALM laser catch microdissection (LCM) platform (Carl Zeiss Ltd., Cambridge, UK) based on the brand’s protocols. This become carried out to enable affirmation of cell-category selected changes in miRNA expression52. total RNA turned into in consequence extracted the usage of the RNeasy FFPE equipment (Qiagen, Hilden, Germany) following the company’s protocol. recommended consent changed into bought from all patients and ethical approval was obtained from the Camden & Islington REC, London (09/H0722/seventy seven) within the UK.

LNA-primarily based microRNA in situ hybridization

sufferers: The purpose of this test changed into to look at expression of miR-100 and miR-125b in human PDAC tissue samples by LNA-based mostly miRNA ISH. The test blanketed formalin-fixed paraffin embedded (FFPE) tumor specimens from a hundred PDAC patients (all resectable Stage IIB) organized on 4 tissue-microarrays (TMA), every containing 25 patient sample. For each affected person tumor there were 4 cores (1.5 mm diameter) to be able to avoid intra-tumoral heterogeneity and most appropriate symbolize the tumor. Hematoxylin and eosin (H&E) staining become performed on all samples prior to processing for the ISH evaluation as a way to verify tumor histology. patients underwent surgery for PDAC at the school of Pisa, Italy throughout 2005–2010 and had been closely adopted-up. none of the patients bought neo-adjuvant chemotherapy, but all bought adjuvant chemotherapy. complete clinicopathological, observe-up and recurrence facts have been available from a prospectively maintained database.

LNA probes: DNA oligonucleotides with approximately 30% Locked Nucleic Acid (LNA) substitutions53 for the total size miRNA had been used: miR-a hundred-5p (expected Tm ~ eighty five °C; goal sequence CACAAGTTCGGATCTACGGGTT; 32% LNA) and miR-125b-5p (expected Tm ~ eighty five °C; goal sequence TCACAAGTTAGGGTCTCAGGGA; 27% LNA) (Exiqon, Vedbaek, Denmark). in addition, a probe selected for U6 snRNA (ACGAATTTGCGTGTCATCCTT; anticipated Tm ~ 83 °C; 29% LNA) become used as fantastic control, and a 21-mer scrambled probe with a random sequence (TGTAACACGTCTATACGCCCA; predicted Tm ~ 87 °C; 33% LNA) having no widely used complementary sequence goal amongst human transcripts performing MegaBLAST search at NCBI GenBank, was included as terrible control (Exiqon, Vedbaek, Denmark). All LNA oligos had been digoxigenin (DIG)-labeled on the 5′- and three′-ends except the U6 probe, which was best 5′-end labeled.

In situ hybridization: five μm-thick paraffin TMA sections have been hooked up on tremendous frost + glass slides and deparaffinized. ISH for miRNA detection turned into carried out using a miRCURY LNA miRNA ISH equipment (Exiqon, Vedbaek, Denmark) as previously described54 with few modifications. For optimization, miR-one hundred and miR-125b probe concentrations and proteolytic pre-medicine had been evaluated on four separate significant FFPE PDAC sections. A proteinase-k (PK) pre-medication of 20 μg ml−1 and probe awareness of 30 nM have been chosen for subsequent TMA analyses.

photograph analysis and quantification: pictures have been obtained the usage of a 20 × and 40 × targets with a Zeiss AxioScan. For miRNA quantification, right here histologically stained buildings had been differentiated: blue areas comparable to the hybridization signal, crimson area similar to the crimson nuclear stain, pink areas comparable to blue ISH signal overlaying nuclear pink stain. Scoring of ISH changed into performed semi-quantitatively based on staining intensity: 0, absent; 1+, weak (seen at 200 × magnification); 2+, reasonable (seen at a hundred × magnification); three+, effective (seen at 40 × magnification). additionally, localization of miRNA sign in both the tumoral and stromal booths changed into assessed. thus, 3 parameters had been received from graphic analysis and reflecting relative miRNA expression levels have been regarded: (1) the stained area, (2) the staining depth and (three) the variety of effective cells. This tissue slide-based miRNA ISH therefore offered qualitative (tumor compartment) and semi-quantitative (expression ranges) records.

Semi-quantitative ISH score: in an effort to accurately describe the extent of ISH staining of a tumor core, the diploma of ISH staining in every compartment (tumor or stromal cells) became measured for every miRNA. They captured the percent of cells stained at every intensity level and then used an depth scale starting from 0 for no staining, to 3+ for probably the most excessive staining. This tips was used to calculate a variable ISH rating, extra continuous than comfortably fantastic versus negative. This ISH rating for each patient tumor or stromal compartment turned into calculated using the mean depth plus % of cells of the 4 cores. The suggest ISH rating for all patients changed into then used to dichotomize patient cores into these with excessive or low miRNA expression in the cellular compartments.

Statistical evaluation: MiRNA ranges in tumor and stromal booths had been in comparison with the pupil’s t-look at various (2-tailed). sufferers demise from PDAC after surgical resection had been regarded as “demise activities” and this changed into used to calculate the OS evaluation. Date of death was obtained from the health center facts, household doctor or Italian Civil Registration. patients who have been classified as having an experience in the calculation of DFS experienced native tumor recurrence within the type of both local or distant metastasis. high and low miRNA ISH rating, the usage of the imply price as a cut-off, changed into then used to associate miRNA expression with survival effects following pancreaticoduodenectomy (PD) surgical procedure (i.e. from date of surgical procedure to this point of demise, or date of disease progression). Survival curves have been constructed using the Kaplan-Meier method and alterations in survival had been compared by using log-rank verify. All statistical analyses had been carried out the use of SPSS 20.0 (IBM, SPSS). P values ≤0.05 had been considered big.

information availability

The accession number for the Nanostring, RNA-seq, RIP-seq, and ChiP-seq facts pronounced in this paper is GEO: GSE88759. All other closing records can be found within the Article and Supplementary files, or accessible from the authors upon request.


BREATH: web-primarily based Self-administration for Psychological Adjustment After primary Breast melanoma—consequences of a Multicenter Randomized controlled Trial

examine Design and contributors

The BREATH examine protocol has been published somewhere else.28 They carried out a multicenter, randomized, managed, parallel-community trial to consider the efficacy of an internet-based mostly self-management intervention in facilitating psychological adjustment amongst BCSs. One institution medical institution and five regional hospitals within the Netherlands participated (Fig 1). female BSCs have been eligible if they had a histologically proven malignancy of the breast and had completed healing-intent basic remedy (defined as surgery plus adjuvant chemotherapy and/or radiotherapy) 2 to four months before the baseline evaluation. Participant qualities are listed in desk 1.

Table

table 1. Baseline features of examine participants (N = one hundred fifty)

table 1. Baseline qualities of study participants (N = 150)

attribute CAU + BREATH (n = 70) CAU alone (n = 80) No. of patients % No. of sufferers % Age, years     suggest fifty one.44 50.18     SD 8.30 9.15 academic degree*     Low (ISCED 0-1-2) 14 20 13 16     Medium (ISCED three-4-5) 32 46 forty eight 60     high (ISCED 6-7-eight) 24 34 19 24 Marital fame     Married/cohabiting fifty eight eighty three sixty one 76     unmarried five7 56     Divorced 57 10 13     Widowed 2 three four fivetoddlers (sure) 61 87 sixty two seventy six Employment†     Paid work backyard home30 43 32 40     domestic management21 30 18 23     Unemployed three 4 eight 10     ill depart 30 43 42 fifty three     disability assurance act 3 four 56     Voluntary paintings7 10 4 5     pupil 0 0 1 1     Retired 3 4 three 4 remedy type     surgery + chemotherapy + radiotherapy 48 sixty nine 56 70     surgical procedure + chemotherapy 19 27 22 28     surgical procedure + radiotherapy 3 4 2 2 Hormone therapy46 66 53 66 Low distress (GSI ≤ 0.57) fifty one 73 fifty five sixty nine Frequency of information superhighway use‡     each day 24 59 38 73     2 to four instances every week 12 29 917     Weekly or much less 512 510

The local medication group monitored affected person recruitment and eligibility and bought counseled consent. A researcher (S.v.d.B.) contacted members to investigate additional eligibility standards: knowing the Dutch language, entry to the internet, and having an email handle.

examine assessments lined the first 12 months after breast melanoma, with baseline at 2 to four months after completion of basic treatment (T0), and observe-up assessments at 4 (T1), 6 (T2), and 10 (T3) months after baseline. The Radboud tuition medical middle medical evaluate Ethics Committee (file No. 2009/144) and the ethics boards of native collaborating centers permitted the examine (Netherlands Trial Register NTR2935).

Intervention

BREATH (Appendix Fig A1, online best) pursuits re-entry concerns significant to BCSs throughout a hard and fast 16-week modular software for four phases of adjustment to breast cancer (searching back, emotional processing, strengthening, and looking ahead).28 Intervention components (104 complete) are based on cognitive behavioral remedy and encompass suggestions (26 scripts), task (48 projects, or homework), assessment (10 self-checks adopted by automatic feedback), and video (20 issues illustrated with clips extracted from interviews). BREATH is a pure self-assist program with out therapist contact (also referred to as self-administered therapy31). each week, new substances are launched, accompanied via usual electronic mail reminders, in an try and hold or enrich adherence. access to BREATH become withdrawn after 16 weeks.

BCSs receiving CAU on my own did not have entry to BREATH. For both conditions, CAU consisted of visits to an oncologist thrice monthly and psychosocial care on demand or referral. No restrictions were made involving use of cyber web or psychological or different self-support interventions.

results

study assessments and random task were conducted on-line the usage of RadQuest application (Radboudumc clinical Psychology, Nijmegen, the Netherlands). Baseline characteristics were got with a medical guidelines derived from the affected person chart.

ordinary psychological distress turned into assessed with the Symptom checklist-ninety (SCL-90; range, ninety-450), which has good psychometric properties in in shape and patient populations.32 The SCL-90 complete ranking confirmed respectable inner consistency at baseline (Cronbach's α = .ninety seven). For the everyday Severity Index (GSI), which represents the suggest score of all responses, changed merchandise scores (0-four) had been used. customary psychological empowerment became assessed with the cancer Empowerment Questionnaire (CEQ).33 The CEQ presumes that sufferers can derive power from themselves (intrapersonal) and from their social ecosystem (interpersonal). Baseline inside consistency changed into first rate (Cronbach's α = .92).

Secondary results reflected negative adjustment (general34,35 and melanoma-selected distress,36 fatigue,37 helplessness,38 and worry of melanoma recurrence39,40) and advantageous adjustment (self-efficacy,forty one remoralization,forty two own control,forty three first-class of existence,forty four success,forty five re-evaluation,forty five new techniques of dwelling,forty five and valuing life45). For details of secondary effects, see their look at protocol28 (or the legend of table 2 with treatment results).

assistance about self-suggested use of internet and other supplies (individual help, peer assist, rehabilitation help organizations) became amassed at T1. standard information superhighway use become assessed with the query “have you consulted the cyber web for counsel on (gaining knowledge of to are living with) breast melanoma in the past 4 months?” four-month usage records of CAU + BREATH participants were evaluated.forty six For the present document, correlations had been calculated between the mean difference (T0 to T1) in distress and the continuous usage variables of frequency of log-ins, complete period (in minutes), and endeavor (number of intervention add-ons opened).

Random task and protecting

BCSs have been randomly assigned (allocation ratio 1:1) to receive CAU + BREATH or CAU on my own. For each middle, a randomized block design with stratification by hormone therapy changed into generated. After baseline evaluation, a random number generator with variable block sizes of 4, 6, and eight instantly ensured blinded allocation until intervention project. One researcher (S.v.d.B.) counseled contributors about medication task by e mail and become for this reason now not blinded. in one case, a participant become informed an flawed medication project (CAU instead of CAU + BREATH); this participant acquired CAU on my own and her statistics had been analyzed as a result.

Statistical methods

All statistical analyses had been carried out with SPSS 20 (IBM, Armonk, the big apple). The sample measurement calculation for the fundamental outcomes at T1 changed into a hundred and seventy: 128 BCSs (64 in every neighborhood) at 80% vigor for ameliorations between CAU + BREATH and CAU alone with a medium effect measurement of 0.50, plus a 25% dropout price.28 The level of value in the pattern dimension calculation was adjusted to P ≤ .025 to keep the universal chance of category I error at 5%. Inclusion turned into prematurely stopped at 151 contributors, with approval of the ethics committee, as a result of only 5% (7 of 151) of the contributors dropped out at T1.

Statistical effect

The simple speculation changed into that CAU + BREATH could be advanced to CAU by myself in reducing misery and lengthening empowerment, and it changed into established in an intention-to-deal with (ITT) evaluation of statistics for T0 and T1. missing data at T1 have been imputed the usage of ultimate commentary carried forward. The significance of intervention results on primary and secondary results became demonstrated the use of one-method between-groups analyses of covariance with group (CAU + BREATH or CAU on my own) as a fixed component. as a result of members have been no longer preselected before inclusion, the analysis of covariance model corrected for baseline variations. For basic result analyses, baseline misery (SCL-ninety) and empowerment (CEQ) have been used as covariates. For secondary outcomes analyses, baseline rankings of corresponding questionnaires have been used as covariates. interplay outcomes of suggest adjusted alterations between T0 and T1 by means of group are mentioned in table 2, with SEs, significance stage, and 95% CI. effect dimension Cohen's d for unbiased groups became calculated using the pooled standard deviations and unadjusted ability on T1.

Table

desk 2. impact of medication (intention-to-deal with analysis) on primary and Secondary consequences After four Months (N = a hundred and fifty)

Clinically enormous change

Clinically significant change, assessed with the authentic exchange index (RCI) of the GSI, turned into proven in ITT evaluation (T0 to T1). Following Jacobson and Truax,forty seven Schauenburg and Strack48 calculated the GSI cutoff 0.567 to discriminate between low and high misery on the groundwork of normative and psychotherapy samples. The magnitude of improvement (described as RCI ≤ −0.16 for low-misery members and RCI ≤ −0.43 for prime-misery members) or deterioration (RCI ≥ 0.sixteen for low-misery individuals and RCI ≥ 0.forty three for prime-distress members) became assessed the use of one-sided χ2 assessments.33

follow-Up impact

follow-up consequences for fundamental and secondary consequences were evaluated with mixed inside-between repeated-measures evaluation of variance, including facts for participants who accomplished all four assessments (T0 to T3). because members were randomly assigned at every core, middle turned into now not protected as a random effect. Baseline variables have been taken under consideration as inside components in the mannequin. ameliorations between CAU + BREATH and CAU on my own were tested using unbiased samples t exams.

Between August 2010 and March 2012, one hundred seventy ladies had been referred and 151 (89%) underwent random assignment (Fig 1). data assortment become finalized in February 2013. One lady become erroneously enrolled (metastatic disorder) and excluded after the random project,49 leaving a remaining ITT pattern of one hundred fifty BCSs (70 CAU + BREATH, 80 CAU on my own). At baseline, the two agencies did not vary on demographic features (desk 1) and analyze results (table 2). contributors with missing facts at T1 (n = 17) had larger tiers of baseline misery than participants with complete records at T1 (imply difference, 23.57; ninety five% CI, three.eighty two to 43.31; P = .02). ranges of baseline empowerment were an identical (P = .79). missing facts at T1 had been equally dispensed between the two corporations. standard, 124 members (fifty eight CAU + BREATH; sixty six CAU on my own) achieved all four assessments, and their data had been included in follow-up analyses. No metastases or severe ailments have been said right through the examine. One girl become admitted to a psychiatric health facility; this was reported as a serious opposed adventure.

Statistical effect

The reduce in misery at T1 turned into greatly improved in CAU + BREATH participants than in CAU-alone individuals, with a small-to-medium impact measurement (d = 0.33; desk 2). Baseline misery defined fifty three% of the variance in misery at T1 (P < .005). No such change in empowerment became found.

Secondary influence analyses (desk 2) printed that CAU+BREATH led to large improvements in 5 of seven terrible adjustment variables (generic and cancer-particular distress, fatigue, and two worry of melanoma recurrence outcomes) with small-to-medium effect sizes (d = 0.37 to 0.55), and in three of 10 superb adjustment variables (self-efficacy, remoralization, new techniques of living) with small-to-medium effect sizes (d = 0.26 to 0.39).

Clinically gigantic change

greater CAU + BREATH members (39 of 70 [56%]; 95% CI, 44.1 to 66.8) than CAU-on my own individuals (32 of eighty [40%]; 95% CI, 30.0 to fifty one.0) showed a clinically colossal improvement (P = .03). They had hypothesized that extra excessive-distress BCSs would show a clinically tremendous improvement after CAU + BREATH than after CAU on my own, however this become now not the case (10 of 21 [48%]; ninety five% CI, 28.3 to 67.6 v 14 of 27 [52%]; 95% CI, 34.0 to sixty nine.3, respectively; P = .39). post hoc evaluation revealed that there changed into no change within the share of high-distress BCSs displaying clinical deterioration (5 of 21 [24%] v 2 of 27 [7%], respectively; P = .06). Of the low-distress BCSs, extra CAU + BREATH individuals than CAU-on my own contributors showed scientific improvement or no alternate (41 of forty nine [84%]; ninety five% CI, 71.0 to ninety one.5 v 35 of fifty three [66%]; ninety five% CI, 52.6 to 77.three respectively; P = .02). moreover, explorative post hoc analyses of low-distress BCSs revealed that, compared with CAU-on my own members, CAU + BREATH individuals showed extra clinically large development (29 of 49 [59%] v 18 of 53 [34%], respectively; P = .006) and less deterioration (eight of forty nine [16%] v 18 of 53 [34%], respectively; P = .02). The empowerment hypothesis become not validated, as a result of empowerment changed into now not greatly different between CAU + BREATH and CAU by myself.

observe-Up effect

At T2 and T3, misery become enormously reduced despite neighborhood assignment (F[3, 120] = 5.88; P = .001; Fig 2). This changed into additionally actual for the secondary terrible adjustment outcomes of concern of melanoma recurrence (cancer be anxious Scale; F[3, 120] = 5.954; P = .001), fatigue (F[3, 120] = four.40; P = .006), and helplessness (F[3, 120] = eleven.964; P = .000). a major time × community interplay effect became discovered for worry of melanoma recurrence (melanoma be concerned Scale; F[3, 120] = 4.563; P = .005), with CAU + BREATH members reporting less concern than CAU-alone members at T2 (−1.459; ninety five% CI, −2.743 to −0.a hundred seventy five). Of the positive adjustment effects, acceptance tremendously greater in both agencies (ICQ; F[3, 120] = 8.531; P = .000). Time consequences and time × community interactions have been no longer big for all remaining results, together with empowerment (Fig 3).

Use of BREATH and other elements

Use of the BREATH intervention distinctive considerably. Frequency of logins ranged from 0 to forty five, total length ranged from 0 to 2,324 minutes, and undertaking ranged from 0 to 104 intervention add-ons opened. The imply change in distress (SCL-ninety, T0 to T1) changed into no longer correlated with frequency (r = −0.007; P = .96), complete length (r = 0.000; P = 1.00), or endeavor (r = −1.072; P = .55).

At T1, equivalent proportions of women in CAU + BREATH and CAU alone had consulted the web in the old four months on a month-to-month (24% v 34%), weekly (13% v 8%), or day by day (0% v 2%) groundwork, or now not in any respect (61% v fifty eight%). there were also no huge modifications (n = 126; P = .27) between CAU + BREATH and CAU-alone members in the use of particular person aid (eg, psychologist; 12% v 25%, respectively), peer and rehabilitation help companies (14% v 12%, respectively), or mixed particular person and neighborhood help (21% v 13%, respectively). Half of the contributors in both organizations didn't make use of different help (53% v 50%, respectively).

in the beginning of the re-entry phase, four-month entry to BREATH in addition to CAU resulted in a statistically and clinically large misery reduction compared with CAU alone. youngsters, this small-to-medium effect became now not sustained, and ranges of distress have been similar at 6 and 10 months. CAU + BREATH individuals also confirmed a better lessen than CAU-by myself individuals in fear of cancer recurrence, fatigue, and familiar and cancer-related misery. The impact of BREATH on concern of melanoma recurrence changed into sustained right through observe-up. entry to BREATH did not influence empowerment or medical misery improvement in high-misery BCSs. Low-distress BCSs showed a stronger scientific improvement and less deterioration with CAU + BREATH than with CAU by myself.

The RCT become designed based on high-quality necessities (CONSORT for parallel group,50 nonpharmacologic medicine,fifty one and eHealth trials).fifty two,fifty three Statistically and clinically giant adjustments had been evaluated in ITT analyses, with missing records imputed the use of a conservative system (ultimate commentary carried ahead) since the sufferers in their pattern have been expected to improve on misery over the examine period.8,9 The multicenter recruitment method certain referrals from both secondary and tertiary care facilities. The recruitment method, which ensured minimal involvement of the analysis team, and shortage of guidance regarding intervention use and adherence assist the ecological validity of BREATH. in the absence of RCTs evaluating equivalent unguided internet-based interventions for BCSs, the effect dimension of BREATH is consistent with that of two contemporary meta-analyses of guided face-to-face (effect size latitude 0.26 to 0.38)54 and internet-primarily based (impact size latitude, 0.17 to 0.21)55 interventions for people with cancer54 and continual somatic situations.fifty five

The secondary results revealed that CAU + BREATH diminished universal and melanoma-selected distress, fatigue, and concern of melanoma recurrence, which may additionally replicate the multicomponent nature of distress in melanoma sufferers.fifty six The clinical relevance of those consequences has to be addressed in future research.

CAU + BREATH didn't vastly alternate empowerment relative to the impact of CAU on my own and had inconsistent effects on the secondary effective adjustment variables. The examine of advantageous adjustment is a brand new research enviornment and poses distinctive challenges. despite the fact new fashions of survivorship care stress affected person empowerment,fifty seven,fifty eight there is not any consensus about the empowerment assemble. as a result, high-quality adjustment questionnaires, such because the CEQ, are new, however no longer greatly validated in BCSs and absence counsel on sensitivity to trade. moreover, in psycho-oncology, aid-oriented therapeutic models59 are missing, and interventions are historically aimed at diminishing deficits in its place of enhancing strengths. it is feasible that BREATH doesn't consist of a ample variety of empowerment modules (handiest four of the sixteen weekly modules focused empowerment).

effects should still be considered with warning for a number of reasons. per the scarce literature on net-primarily based interventions for melanoma sufferers,60 BREATH didn't have a sustained effect on misery. This could be as a result of access to the net web site became for 4 months only. besides the fact that children the limited access enabled accurate postintervention assessments, on reflection it may had been improved to enable participants to continue access, specially as a result of counsel commonly continues to be attainable with different psychoeducational interventions or self-assist books. a further cause of lack of a sustained impact may well be the small-to-medium effect dimension, which may no longer had been sufficient to make amends for the natural course of emotional restoration. The missing-at-random assumption for imputation changed into violated. BCSs with missing data for the four-month evaluation had drastically higher degrees of distress at baseline. besides the fact that children now not gigantic, more high-distress BCSs within the CAU + BREATH community confirmed a scientific deterioration. This leads to a cautious interpretation of the results concerning excessive-misery BCSs, and means that these ladies may wish a more intensive intervention than BREATH.

The restrained facts on BREATH use means it isn't possible to draw firm conclusions about how commonly the intervention should still be used to have an effect. extra investigations with bigger samples, mediation analyses, or usage pattern are vital to gain insight into determinants of intervention use and to study a probable dose-response relationship.sixty one statistics for BCSs who declined to participate were now not recorded. even though it become not feasible to recruit sufferers consecutively, their pattern seemed homogeneous and representative, since the suggest age, medicine type, training, and work condition of members had been comparable to these of other experiences evaluating the Dutch breast cancer population.three,9,62,63 The analyze pattern also proved representative in regards to psychological functioning all through the primary 12 months after medicine. As in different experiences of patients with breast8,9 or other64 melanoma, most patients experienced low ranges of misery (n = 102 [68%]).

To the better of their knowledge, here is the first RCT to exhibit an extra effect of a self-administration intervention specially designed to support BCSs in the yr after treatment completion. despite the fact small, the simple effect on distress become statistically mighty and clinically relevant. in addition, the intervention does not necessarily require a lot of person commitment. Future analysis should still focus on replicating the current findings, the use of extra valid questionnaires for the effective adjustment variables, and evaluating the observe-up effect past four months of access. The magnitude of the impact in BCSs with low and excessive distress may still be investigated additional. BREATH demonstrated its potential as a possible first step in a matched supportive care model providing evidence-primarily based and easily accessible re-entry care.

1. F Mullan : Re-Entry: The tutorial needs of the cancer survivor health Educ Q 10: 88– 94,1984 suppl Medline, Google scholar2. AL Stanton : What happens now? Psychosocial look after melanoma survivors after medical treatment completion J Clin Oncol 30: 1215– 1220,2012 link, Google student3. E Pauwels, C Charlier, I De Bourdeaudhuij , etal: Care needs after fundamental breast cancer medicine: Survivors' linked sociodemographic and scientific qualities Psycho-Oncology 22: one hundred twenty five– 132,2013 Crossref, Medline, Google studentfour. JD Allen, S Savadatti, AG Levy : The transition from breast cancer ‘patient’ to ‘survivor.’ Psycho-Oncology 18: 71– 78,2009 Crossref, Medline, Google student5. EM Arnold : The cessation of melanoma treatment as a disaster Soc Work fitness Care 29: 21– 38,1999 Crossref, Medline, Google student6. PA Ganz, L Kwan, AL Stanton , etal: actual and psychosocial restoration within the 12 months after simple remedy of breast cancer J Clin Oncol 29: 1101– 1109,2011 link, Google pupil7. okay Bell, S Ristovski-Slijepcevic : melanoma survivorship: Why labels count J Clin Oncol 31: 409– 411,2013 hyperlink, Google student8. VS Helgeson, P Snyder, H Seltman : Psychological and actual adjustment to breast cancer over 4 years: picking diverse trajectories of alternate fitness Psychol 23: 3– 15,2004 Crossref, Medline, Google scholar9. I Henselmans, VS Helgeson, H Seltman , etal: Identification and prediction of misery trajectories in the first 12 months after a breast cancer prognosis fitness Psychol 29: 160– 168,2010 Crossref, Medline, Google pupil10. R McCorkle, E Ercolano, M Lazenby , etal: Self-management: Enabling and empowering patients dwelling with melanoma as a chronic affliction CA-cancer J Clin 61: 50– sixty two,2011 Crossref, Medline, Google scholar11. NK Aaronson, V Mattioli, O Minton , etal: past remedy – Psychosocial and behavioural concerns in cancer survivorship research and apply Eur J cancer supplements 12: 54– sixty four,2014 Crossref, Google student12. M Afshari, MFA Ghani, NM Radzi : Use of the web with the aid of breast melanoma sufferers Sci Res Essays 6: 3571– 3576,2011 Crossref, Google student13. G Eysenbach : The impact of the internet on melanoma effects CA-cancer J Clin fifty three: 356– 371,2003 Crossref, Medline, Google scholar14. J Fogel, SM Albert, F Schnabel , etal: Use of the internet with the aid of women with breast melanoma J Med internet Res 4: e9,2002 Crossref, Medline, Google scholar15. SA Littlechild, L Barr : the usage of the web for assistance about breast cancer: A questionnaire-based analyze patient Educ Couns 2013 Crossref, Medline, Google pupilsixteen. Y Leykin, SM Thekdi, DM Shumay , etal: cyber web interventions for making improvements to psychological well-being in psycho-oncology: assessment and recommendations Psycho-Oncology 21: 1016– 1025,2012 Crossref, Medline, Google pupil17. DL Berry, F Hong, B Halpenny , etal: electronic self-document assessment for cancer and self-care aid: consequences of a multicenter randomized trial J Clin Oncol 32: 199– 205,2014 hyperlink, Google pupil18. KM chippie, SA Stoner, k Schmitz , etal: a web stress management workbook for breast cancer J Behav Med 2012 Google scholar19. AL Stanton, EH Thompson, CM Crespi , etal: mission join online: Randomized trial of a web-based mostly application to chronicle the melanoma experience and facilitate communique J Clin Oncol 31: 3411– 3417,2013 hyperlink, Google pupil20. F Ventura, J Ohlen, I Koinberg : An integrative evaluate of supportive e-fitness programs in cancer care Eur J Oncol Nurs 17: 498– 507,2013 Crossref, Medline, Google pupil21. L Beatty, B Koczwara, T Wade : ‘melanoma Coping online’: A pilot trial of a self-guided CBT internet intervention for cancer-related distress Electron J Appl Psychol 7: 17– 25,2011 Google pupil22. J Duffecy, S Sanford, L Wagner , etal: mission onward: An ingenious e-health intervention for melanoma survivors Psycho-Oncology 22: 947– 951,2013 Crossref, Medline, Google student23. DH Gustafson, R Hawkins, S Pingree , etal: impact of computer support on more youthful ladies with breast cancer J Gen Intern Med 16: 435– 445,2001 Crossref, Medline, Google pupil24. MT Hoybye, SO Dalton, I Deltour , etal: effect of information superhighway peer-help businesses on psychosocial adjustment to melanoma: A randomised analyze Br J melanoma 102: 1348– 1354,2010 Crossref, Medline, Google scholar25. JE Owen, JC Klapow, DL Roth , etal: Randomized pilot of a self-guided cyber web coping neighborhood for ladies with early-stage breast melanoma Ann Behav Med 30: 54– 64,2005 Crossref, Medline, Google student26. S Lepore, J Buzaglo, M Lieberman , etal: evaluating regular versus prosocial internet assist groups for sufferers with breast melanoma: A randomized managed trial of the helper remedy precept J Clin Oncol 32: 4081– 4294,2014 link, Google pupil27. AM Ryhanen, M Siekkinen, S Rankinen , etal: The results of cyber web or interactive computing device-primarily based affected person education in the container of breast cancer: a systematic literature assessment affected person Educ Couns seventy nine: 5– 13,2010 Crossref, Medline, Google scholar28. SW van den Berg, MF Gielissen, PB Ottevanger , etal: rationale of the BREAst cancer e-fitness [BREATH] multicentre randomised controlled trial: a web-primarily based self-administration intervention to foster adjustment after healing breast melanoma by way of lowering distress and lengthening empowerment BMC cancer 12: 394,2012 Crossref, Medline, Google student29. F Lazarus, S Folkman : Stress, appraisal, and coping 1984 manhattan, ny Springer Google student30. MA Andrykowski, E Lykins, A Floyd : Psychological fitness in melanoma survivors Semin Oncol Nursing 24: 193– 201,2008 Crossref, Medline, Google student31. MG Newman, LE Szkodny, SJ Llera , etal: A evaluation of know-how-assisted self-aid and minimal contact treatment options for anxiety and depression: Is human contact indispensable for therapeutic efficacy? Clin Psychol Rev 31: 89– 103,2011 Crossref, Medline, Google scholar32. WA Arindell, JHM Ettema : Handleiding bij een multidimensionele psychopathologie-indicator: Symptom guidelines SCL-ninety. (Herziene uitgave) 2003 Lisse, the Netherlands Swets & Zeitlinger Google scholar33. SW van den Berg, FK van Amstel, PB Ottevanger , etal: The melanoma Empowerment Questionnaire: Psychological empowerment in breast cancer survivors J Psychosoc Oncol 31: 565– 583,2013 Crossref, Medline, Google student34. MA Tuinman, SM Gazendam-Donofrio, JE Hoekstra-Weebers : Screening and referral for psychosocial distress in oncologic observe: Use of the distress thermometer melanoma 113: 870– 878,2008 Crossref, Medline, Google scholar35. A Vodermaier, RD Millman : Accuracy of the hospital anxiety and melancholy Scale as a screening tool in cancer patients: a scientific evaluate and meta-evaluation guide Care melanoma 19: 1899– 1908,2011 Crossref, Medline, Google pupil36. E van der Ploeg, TT Mooren, RJ Kleber , etal: assemble validation of the Dutch version of the have an impact on of experience Scale Psychol examine sixteen: 16– 26,2004 Crossref, Medline, Google pupil37. MFM Gielissen, S Verhagen, F Witjes , etal: results of cognitive conduct therapy in severely fatigued disease-free cancer patients in comparison with sufferers waiting for cognitive conduct therapy: A randomized managed trial J Clin Oncol 24: 4882– 4887,2006 link, Google pupil38. AWM Evers, FW Kraaimaat, W van Lankveld , etal: beyond negative considering: The disorder cognition questionnaire for chronic ailments J talk to Clin Psychol sixty nine: 1026– 1036,2001 Crossref, Medline, Google scholar39. JAE Custers, SW van den Berg, HWM van Laarhoven , etal: melanoma be anxious Scale: Detecting worry of melanoma recurrence in breast cancer survivors melanoma Nurs 37: E44– E50,2014 Crossref, Medline, Google studentforty. P Servaes, S Verhagen, HW Schreuder , etal: Fatigue after medicine for malignant and benign bone and smooth tissue tumors J pain Symptom manipulate 26: 1113– 1122,2003 Crossref, Medline, Google pupilforty one. P Servaes, S Verhagen, G Bleijenberg : Determinants of persistent fatigue in disorder-free breast cancer sufferers: A move-sectional examine Ann Oncol 13: 589– 598,2002 Crossref, Medline, Google scholar42. W Vissers, GPJ Keijsers, WM van der Veld , etal: development of the Remoralization Scale: An extension of modern psychotherapy influence dimension Eur J Psychol evaluation 26: 293– 301,2010 Crossref, Google scholarforty three. I Henselmans, R Sanderman, VS Helgeson , etal: personal control over the remedy of breast melanoma: Adaptiveness, underlying beliefs and correlates Psycho-Oncology 19: 525– 534,2010 Crossref, Medline, Google scholarforty four. NK Aaronson, S Ahmedzai, B Bergman , etal: the eu organization for research and treatment of melanoma QLQ-C30: a quality-of-lifestyles instrument to be used in overseas scientific trials in oncology J Natl melanoma Inst eighty five: 365– 376,1993 Crossref, Medline, Google pupilforty five. JS Boot, C Holcombe, P Salmon : superb adjustment to breast melanoma: building of a disorder-specific measure and evaluation of girls diagnosed from 2 weeks to five years Psycho-Oncology 19: 1187– 1194,2010 Crossref, Medline, Google studentforty six. SW van den Berg, EJ Peters, JF Kraaijeveld , etal: utilization of a ordinary net-based mostly self-management intervention for breast melanoma survivors: Substudy analysis of the BREATH trial J Med internet Res 15: e170,2013 Crossref, Medline, Google scholarforty seven. NS Jacobson, P Truax : medical significance: A statistical method to defining significant change in psychotherapy research J check with Clin Psychol 59: 12– 19,1991 Crossref, Medline, Google pupil48. H Schauenburg, M Strack : Measuring psychotherapeutic trade with the Symptom checklist SCL ninety R Psychother Psychosom 68: 199– 206,1999 Crossref, Medline, Google pupil49. D Fergusson, SD Aaron, G Guyatt , etal: post-randomisation exclusions: The intention to treat precept and excluding patients from evaluation BMJ 325: 652– 654,2002 Crossref, Medline, Google scholar50. D Moher, S Hopewell, KF Schulz , etal: CONSORT 2010 clarification and elaboration: updated guidelines for reporting parallel neighborhood randomised trials BMJ 340: c332,2010 Crossref, Medline, Google student51. I Boutron, D Moher, DG Altman , etal: Extending the CONSORT remark to randomized trials of nonpharmacologic remedy: explanation and elaboration Ann Intern Med 148: 295– 309,2008 Crossref, Medline, Google pupil52. TB Baker, DH Gustafson, B Shaw , etal: Relevance of CONSORT reporting criteria for research on eHealth interventions patient Educ Couns 81: S77– S86,2010 Crossref, Medline, Google pupilfifty three. G Eysenbach, C-E Grp : CONSORT-EHEALTH: improving and standardizing contrast stories of net-based and cell health interventions J Med web Res 13: e126,2011 Crossref, Medline, Google pupilfifty four. H Faller, M Schuler, M Richard , etal: effects of psycho-oncologic interventions on emotional misery and nice of existence in grownup sufferers with melanoma: Systematic evaluation and meta-evaluation J Clin Oncol 31: 782– 793,2013 link, Google scholar55. S van Beugen : information superhighway-based mostly cognitive behavioral remedy for sufferers with chronic somatic situations: A meta-analytic assessment J Med information superhighway Res 16: e88,2014 Crossref, Medline, Google scholar56. JC Holland, B Andersen, WS Breitbart , etal: misery management J Natl Compr Canc Netw 8: 448– 485,2010 Crossref, Medline, Google scholarfifty seven. L Hood, SH pal : Predictive, customized, preventive, participatory (P4) cancer medicine Nat Rev Clin Oncol eight: 184– 187,2011 Crossref, Medline, Google pupil58. G Pravettoni, A Gorini : A P5 melanoma medicine method: Why personalized medication cannot ignore psychology J Eval Clin Pract 17: 594– 596,2011 Crossref, Medline, Google scholar59. S Priebe, S Omer, D Giacco , etal: useful resource-oriented therapeutic models in psychiatry: Conceptual overview Brit J Psychiat 204: 256– 261,2014 Crossref, Medline, Google scholar60. H McAlpine, L Joubert, F Martin-Sanchez , etal: a scientific assessment of forms and efficacy of online interventions for melanoma sufferers patient Educ Couns ninety eight: 283– 295,2015 Crossref, Medline, Google pupil61. L Donkin, IB Hickie, H Christensen , etal: Rethinking the dose-response relationship between usage and result in an internet intervention for depression: Randomized managed trial J Med cyber web Res 15: sixty seven– eighty,2013 Crossref, Google studentsixty two. C Hinnen, AV Ranchor, R Sanderman , etal: course of misery in breast cancer patients, their companions, and matched manage couples Ann Behav Med 36: 141– 148,2008 Crossref, Medline, Google studentsixty three. PJ Vos, AP Visser, B Garssen , etal: consequences of delayed psychosocial interventions versus early psychosocial interventions for girls with early stage breast melanoma affected person Educ Couns 60: 212– 219,2006 Crossref, Medline, Google scholar64. J Dunn, SK Ng, W Breitbart , etal: fitness-related excellent of lifestyles and life delight in colorectal cancer survivors: Trajectories of adjustment fitness Qual life consequences 11: forty six,2013 Crossref, Medline, Google scholar
We provide dumps for following vendors
BEA | Pegasystems | HR | Certiport | APC | 3COM | Genesys | EMC | Enterasys | APTUSC | mySQL | ECDL | PayPal | Salesforce | Veritas | BICSI | ISEB | CA-Technologies | Cloudera | Brocade | GuidanceSoftware | SDI | LSI | RedHat | Axis | CyberArk | CIPS | Healthcare | Ericsson | Intel | Magento | H3C | LSAT | ARM | SUSE | AIIM | Food | PsychCorp | ISC2 | Teacher-Certification | FCTC | APA | RES | Business-Objects | LPI | ITEC | IRS | ISACA | Juniper | Hospitality | The-Open-Group | iSQI | Citrix | Dassault | Siemens | Trainers | Android | Network-General | Amazon | Alfresco | CompTIA | ICAI | Sair | IELTS | PMI | SCP | DMI | McAfee | Quality-Assurance | Acme-Packet | HDI | SOA | IEEE | IQN | Nortel | HRCI | Zend | BlackBerry | IFPUG | ESPA | ASQ | ADOBE | Hortonworks | Liferay | FSMTB | P&C | F5-Networks | Worldatwork | VCE | AccessData | OMG | Altiris | Esri | NVIDIA | ICDL | Informatica | Autodesk | Tibco | BlueCoat | Prince2 | CWNP | Riverbed | GRE | PARCC | QlikView | SpringSource | Hyperion | IBM | Social-Work-Board | LEED | AppSense | PostgreSQL-CE | Exin | Huawei | ACI | Symantec | NCLEX | College-Board | Cisco | Business-Tests | ACT | NI | CPP-Institute | Logical-Operations | Counselor | CIW | Lotus | ExtremeNetworks | GIAC | Financial | Misc | AXELOS | Filemaker | TIA | PTCB | CheckPoint | ISM | SANS | HIPAA | IISFA | NetworkAppliance | Polycom | TruSecure | QAI | Banking | USMLE | ASIS | PRMIA | GMAT | ComputerAssociates | ASTQB | NIELIT | Mile2 | Google | See-Beyond | Oracle | RSA | SASInstitute | Palo-Alto | SCO | Consultant | ACFE | Military | NCEES | NCIDQ | Cognos | Legato | ECCouncil | Apple | SAT | Real-Estate | Sybase | Motorola | APICS | DELL | AFP | IAHCSMM | Fujitsu | IAAP | Veeam | Vmware | Hitachi | Certification-Board | Arizona-Education | PEOPLECERT | Novell | SPSS | Trend | ACSM | Nokia | Fortinet | XML-Master | GAQM | AICPA | DRI | Microsoft | SAP | Real Estate | HP | Alcatel-Lucent | IBQH | IIBA | ExamExpress | RACC | ISA | Sun | McData | Medical | Avaya | Isilon | Wonderlic | Admission-Tests | American-College | NBSTSA | SNIA | CIDQ | CSP | Foundry | Aruba |
braindumps.com | brain P2020-012 dumps - ccna dumps, mcitp braindumps, certification exam dumps | new certification dumps: ccna dumps, cisco brain dumps, mcsa, mcse, mcitp it exam dumps. pass exams with braindumps questions answers, download testing engines | dumps, braindumps, mcse, exam, dump, brain, ccna, engine, testing, simulator, braindump, download
getfreedumps.com | request dumps | |
cardingmafia.ws | carding forum - carding - credit cards - P2020-012 dumps - tracks - laptops shipping - bank accounts - western union transfer | carding forum, carding, carders, western union transfer, illegal credit cards, credit card, cc, tracks, dumps, pin, dell alienware, hacking, botnet, security, paypal, bank account, login, porn xxx, passwords, email, leads, liberty reserve, ukash, webmoney, shipping laptops, cheap electronics, bank transfer | bank, credit, transfer, carding, passwords, email, account, porn, leads, login, reserve, cheap, electronics, laptops, shipping, ukash, webmoney, liberty, botnet, illegal
devil-group.com | devil-group largest hacker community | yahoo booters | cracking tools | get free rdp | carding forum | wu transfer | free paypal transfer | free bank transfer | bank transfer | credit card hacking | credit card hackers | hacked paypal accounts | carding tutorials | cc checker free | online cc checker | bin checker | cheap shipping | P2020-012 dumps with pin | P2020-012 dumps 101 | free P2020-012 dumps and credit cards | tools, accounts, window, carding, yahoo, ddos, spaming, hacking, xbox360, movies, music, paltalk, fraudcc, true, carders, viprasys, booters, hackingworld, cchacked, ccvhacked
darknetforums.com | carding forum,carders forum,hacked credit card forum,cvv hack forum,hackers forum cvv,russian hackers forum | alphabay , grams , alphabay url , alphabay market , dream market , alph bay , darknet market , grams url , nucleus market , darknet , safe-mail.net , abraxas market , grams search enguine , virvox , darknet markets , safemail , deepdotweb , tor vpn , deepdotweb alphabay , buy bitcoin with paypal , how to buy bitcoins with paypal , ramp , alpha bay market , darknetmarkets , tor over vpn , darknet sites , agora market , nucleus marketplace , how to use pgp , agora marketplace , pandora black market , best darknet sites, darknet website list, black market websites list, black market websites 2015, best darknet market, dream market reddit, amazon dark, deep web markets reddit, , rdp working list 2015 , rdp working list 2016 , credit card hack forum , paypal receipt generator , carding amazon , amazon receipt generator , non vbv sites , darknet forums , darknet market , alphabay market , credit card P2020-012 dumps , free credit cards , carding forum , carders forum , wu transfer , cc P2020-012 dumps , legit | forum, hackers, carding, sites, best, dumps, darknet, hacking, with, carders, credit, card, free, inbox, checker, mailer, hacker, market, account, bank
passleader.com | passleader | it exam P2020-012 dumps with pdf and vce, 100% pass guaranteed! | passleader.com - no.1 provider of cisco, microsoft, comptia, vmware, oracle certification exams. free try new exam questions, 100% money back guarantee! | test, exam, dumps, software, braindump, practice, questions, engine, passleader
crackingweb.com | crackingforum - crackingweb | cracking downloads, cracking tutorial, free premium accounts, sentry mba | cracking, hack, crack, programming, proxies, program, combos, spotify, hacking, configs, software, development, sentry, netflix, dumps
siph0n.net | siph0n - exploits : leaks : P2020-012 dumps : papers : hashes | siph0n exploits and leaks database | exploits, exploit, security, code, dumps, local, linux, remote, root, windows, shellcode, ezines, zines, papers, articles, zeroday, latest, 0day, hashs, hashes
ecubin.com | original ecu files | car computer software - dumps, bins | find original ecu file by make, model and engine or hw and sw number | unit, control, electronic, chip, database, ecubin, engine, model, make, eprom, file, dump, maps, flash, bench, graphs, original
istqb.guru | istqb guru | istqb guru - get all your istqb questions answered, sample papers, sure pass istqb P2020-012 dumps 2015, tips & tricks, study guide & preparation materials for software testing certification |
certmagic.com | all in one solution of certification exams | certmagic provides all the solutions of microsoft exams, vmware certification, ibm tests, oracle braindumps. P2020-012 dumps and test questions are available to clear exam. | certification, oracle, braindumps, cisco, tests, exams, vmware, microsoft
pass4sures.com | unlimited access to 3500+ pass4sure exams q & a for $149.00 only | for only $149.00 you will have unlimited access of all pass4sure exams certification including, cisco, vmware, comptia, microsoft, oracle, and many more. | pass4sure, questions, exam, study, guide, download, dumps, certification, braindumps, pass4sures, reviews
dumps4free.com | dumps4free - free pdf & vce dumps | get free pdf and vce P2020-012 dumps from dumps4free. latest and updated ones. |
kickexam.com | kick exam - clear your exams easily | download upwork skill test P2020-012 dumps include all questions and answers. pass exams with top 1%, 5% or 10% position and get high levels expert / master. |
dumpscollection.net | welcome to P2020-012 dumps collection - free P2020-012 dumps collection | the P2020-012 dumps collection includes microsoft cisco comptia ibm oracle and many more. download a free dumps. |
exams4sure.com | exams4sure real exam dumps | get real exam P2020-012 dumps with 100% passing guarantee. exams4sure real exam questions answers pdf and test engine software. |
itexamfun.com | itexamfun goal help you get passed in all it certification exams first attempt. high pass rate and success rate. | like teat king, pass4sure, itexamfun provides latest real certification practice questions and answers and help you pass your it exam easily. | exam, practice, certification, test, dumps, provider, cert, material
xgallery-dump.com | xgallery-dump.com free adult dumps | free viewing and sharing of adult pictures |
elearningexams.com | free microsoft exams learning center and questions answers P2020-012 dumps - exams study material | all microsoft exams questions answers free practice test sample questions available. your ultimate source of study. |
sellcvvdumps.com | sell cvv (cc) , P2020-012 dumps track 1 track 2 with pin | verified seller | sell cvv good and fresh all country, sell cvv2 P2020-012 dumps , sell P2020-012 dumps track 1 track 2 with pin , buy cvv us uk ca aus eu fr jp eu china , sell bank login cvv fullz |
snesmusic.org | snesmusic.org ~ your place for all snes music needs | the world's most complete and grand archive of super nintendo music, spc700 P2020-012 dumps in .spc format. also a huge database of game information, reviews and more spanning many hundreds of titles. | music, nintendo, super, game, famicom, video, snes
dumps4shared.com | miscrosoft mcse,mcsa,mcse,mcitp,mcts certification exam dumps | free miscrosoft certification study guides, free practice exams, tutorials and more |
crackingcrew.com | crackingforum | sentry mba configs | vertex | dorks | sentry mba configs , hacking, cracking, software development, netflix, spotify, dumps, proxies, dorks, crack, hack, programming, program, hq combos , cracking | cracking, hack, crack, programming, proxies, program, combos, spotify, hacking, configs, software, development, sentry, netflix, dumps
examsokay.com | examsokay- leading provider on all it certification real exam practice and test questions and answers. | examsokay exam materials help you pass all corresponding it exams: microsoft, cisco, ibm, sun, juniper, hp, a+, ccna, mcitp, comptia, oracle and all others. | exam, practice, certification, test, dumps, provider, cert, material
examcollectionuk.com | free vce P2020-012 dumps collection - examcollection | exam collection is the place to collect free vce exams you needed. unlimited download of vce P2020-012 dumps in uk. | braindumps, dumps, examcollection
pass4surebraindumps.com | pass4sure braindumps | latest pass4sure braindumps question and answers, free P2020-012 dumps discount coupon and instant download. | real, exam, answers, question, braindumps, dumps, pass4sure
vitavpks.com | ps vita free games | download free ps vita games | game, vita, wololo, playstation, henkaku, pspiso, dumps, free, download, vitamin
siph0n.in | siph0n - exploits : leaks : P2020-012 dumps : papers : hashes | siph0n exploits and leaks database | exploits, exploit, security, code, dumps, local, linux, remote, root, windows, shellcode, ezines, zines, papers, articles, zeroday, latest, 0day, hashs, hashes
pdfdumps.us | pdf dumps | |
buycvvdumps.com | verified legit 100% | sell cvv - P2020-012 dumps track - wu transfer | best P2020-012 dumps vendor, buy cvv P2020-012 dumps verified seller, paypal account, carding wu transfer, credit card pin, bank login cvv fullz, track 1 2, legit hacker, cc us uk | dumps, track, cvv2, creditcard, transfer, dump, good, vendor, legit, trusted, fresh, sell, online, shop, fullz, paypal, seller, account, best
chemtrailsplanet.net | chemtrails: the exotic weapon | _____________________ climate scientist explains how rainfall is inhibited to produce drought when jet aircraft dump aerosols into the atmosphere _____________________ telescope provides clear evidence of jet aircraft aerosol P2020-012 dumps that produce artificial climate change essential weather and tracking tools debunking the chemtrail debunkers search this website with custom google search archive.org with custom google youtube |
prep4sure.com | prep4sure - best it training and certification computer-based-training online resources | stand out from the it crowd with prep4sure - take advanced training classes and build your it certification career - best technical certifications preparation. | certification, sure, questions, exam, dumps, answers, pass4sure, prep, real, question, test, pass, questionsdumps, original, provider, brain, material, online, training, study
realbraindumps.com | real braindumps with 100% exam passing guarantee | buy real exam P2020-012 dumps from realbraindumps and pass your exam with confidence. |
hpdumps.com | braindump2go free hot it exam P2020-012 dumps collection! | collection of latest braindump2go hot exam P2020-012 dumps questions! | microsoft, exam, questions, braindump2go, dumps
sanidumps.com | sanidumps: rv dumps, sani station, dump points comprehensive directory | rv dump stations, sani dump stations, dump points - comprehensive directory of recreational vehicle dump sites. when rvs have to go... |
exam-labs.com | examdeal | it certification materials | videos | study guides. | examdeal provides latest it certification questions, video tutorials, study guides on microsoft mcsa 2012, mcse 2012, ccna 200-120, ccnp, itil v3, comptia a+ and many more. first exam material provider in nigeria since 2008. | dumps, latest, ccna, itil, vmware, examdeal, other, oracle, microsoft, vcp5, mcitp, 2012, video, mcsa, tutorial, dumpe, comptia, citrix, redhat, novell
prepaway.com | examdeal | it certification materials | videos | study guides. | examdeal provides latest it certification questions, video tutorials, study guides on microsoft mcsa 2012, mcse 2012, ccna 200-120, ccnp, itil v3, comptia a+ and many more. first exam material provider in nigeria since 2008. | dumps, latest, ccna, itil, vmware, examdeal, other, oracle, microsoft, vcp5, mcitp, 2012, video, mcsa, tutorial, dumpe, comptia, citrix, redhat, novell
cop.su | cop.su - ??????? ?????. ???????, ????????, ??????, ?????????, ?????? ??? ??????. | ???????-?????, ???????? | dumps, carding, fraud, forum, visa, mastercard, cashout, carder, selling
examdeal.net | examdeal | it certification materials | videos | study guides. | examdeal provides latest it certification questions, video tutorials, study guides on microsoft mcsa 2012, mcse 2012, ccna 200-120, ccnp, itil v3, comptia a+ and many more. first exam material provider in nigeria since 2008. | dumps, latest, ccna, itil, vmware, examdeal, other, oracle, microsoft, vcp5, mcitp, 2012, video, mcsa, tutorial, dumpe, comptia, citrix, redhat, novell
newcerts.com | newcerts 100% accurate exam brain P2020-012 dumps with latest update. download the free demo to check first. | newcerts has the latest it exam study practice and real exam questions and answers which will help you pass it exams and get it certifications easily. | exam, certification, dump, brain
cardersforum.ws | carders forum - carding forum -hacking forum | cardersforum.ws | professionals hackers & carders forum. world's no1 legit verifed carding forum. you can find free ccv paypal P2020-012 dumps accounts P2020-012 dumps hacking & cracking tools | carding, forum, tools, blackhat, socks5, shipped, hacking, legit, carders, private, logs
killexams.com | pass certification exam with killexams braindumps! best cheat sheets | download kill exams cheat sheet with shortest questions and answers, actual brain P2020-012 dumps and exam simulator to pass the exam at first attempt with money back guarantee. | braindump, exam, cheat, pass4sure, dump, brain, questions, answers, certification
ccnadump.com | pass4sure|braindumps|testking|ensurepass real cisco dumps | ccna, ccent, ccnp, and security help. free and almost-free study guides. | iuwne, icomm, spngn1, iins, dcict, dcicn, spngn2, icnd2, vivnd, icnd1, ccna, routing, cicd, switching, civnd
cardingf.com | cardingf.com - #1 fraud carding forum, top carders forum with legit carders and hackers. real wu, mg and bank transfers. ccv, P2020-012 dumps pin, darknet forum marketplace | best carding forum, carders forum, cvv, credit cards, fraud, paypal, carding tutorials, wu transfer, bank transfer, hacking forum, legit carding, P2020-012 dumps track 1/2, darknet forum, | carding, 2016, free, forum, carders, stealer, dumps, darknet, best, bank, hackers, cheap, forums, money, generator, bitcoin, logins, amazon, websites, card
rdd.pw | rdd.pw - buy P2020-012 dumps shop track1 & track 2 | fresh dumps! buy P2020-012 dumps shop of superior quality. track1 & track 2. | dumps, sell, good, track2, dump, dumpshop, online, fresh, cheap, shop
certificationking.net | braindumps-latest it certification exam brain dump pdf | latest exam questions brain P2020-012 dumps for microsoft, cisco ccna, sun, oracle, novell, ciw, citrix certifications file in pdf format. |
emmchome.com | emmc??-emmc??_?????_full dumps_??????_????????_?????_??????_????_?????_emmc??_??_?????????? - ???? by moorc | emmc???bbs.emmchome.com?????????????????????????????????????up-828??????? up-828e?????????upm-100?? ??epr-box????? ?????????????????? | full, dumps
kitchenpotential.com | kitchen potential: home 820-421 test 820-422 test c2170-008 test c2180-275 test c2180-276 test c4040-123 test jn0-343 test m70-201 test m70-301 test ns0-504 test 70-410 test pw0-204 test 3001 exam P2020-012 dumps 050-720 exam P2020-012 dumps 070-480 exam P2020-012 dumps 070-487 exam P2020-012 dumps 1z0-062 exam P2020-012 dumps 1z0-597 exam P2020-012 dumps 1z0-899 exam P2020-012 dumps 250-310 exam P2020-012 dumps 350-018 exam P2020-012 dumps 400-051 exam P2020-012 dumps 70-178 exam P2020-012 dumps 70-331 exam P2020-012 dumps 70-413 exam P2020-012 dumps 70-465 exam P2020-012 dumps 70-467 exam P2020-012 dumps 70-484 exam P2020-012 dumps 70-485 exam P2020-012 dumps 74-338 exam P2020-012 dumps 74-344 exam P2020-012 dumps 810-420 exam P2020-012 dumps 98-367 exam P2020-012 dumps 7-410 exam P2020-012 dumps 200-120 exam P2020-012 dumps c2010-571 exam P2020-012 dumps c2040-988 exam P2020-012 dumps c4040-226 exam P2020-012 dumps c4120-782 exam P2020-012 dumps cissp exam P2020-012 dumps cpcm exam P2020-012 dumps m70-101 exam P2020-012 dumps mb6-700 exam P2020-012 dumps mb7-701 exam P2020-012 dumps vcad510 exam P2020-012 dumps 3605 exam P2020-012 dumps 7303 exam P2020-012 dumps 000-563 exam P2020-012 dumps 070-337 exam P2020-012 dumps 070-414 exam P2020-012 dumps 070-459 exam P2020-012 dumps 070-460 exam P2020-012 dumps 070-466 exam P2020-012 dumps 070-483 exam P2020-012 dumps 070-685 exam P2020-012 dumps 074-338 exam d | |
allexamdumps.com | all exam dumps | dumps, free dumps, vcp5 dumps, vmware dumps, vcp dumps, vcp4 dumps, vcap dumps, vcdx dumps, cisco dumps, ccna, ccna dumps, ccnp dumps, ccie dumps, itil, exin dumps, | dumps, ccna, ccnp, ccie, exin, itil, cisco, vcap, vcp5, free, vmware, vcp4, vcdx
itexampress.com | latest microsoft, cisco, comptia,oracle,ibm,sun,juniper,hp and all it certification P2020-012 dumps - itexampress | latest {catalog_name} dumps, {catalog_name} certification dumps. hight pass rate, high quality and easy to use - itexampress | dumps, certification, practice, test, exam, latest
it-certification-dumps.blogspot.in | it certification P2020-012 dumps : oracle, ibm and many more | it certification P2020-012 dumps : oracle, ibm and many more | dumps, certification, oracle, cisco, certifications, ocpjp, free, actual, download, scjp, brain
swipe.su | swipe and approved P2020-012 dumps shop | online cc P2020-012 dumps shop P2020-012 dumps cc shop swiped su approved su P2020-012 dumps su best P2020-012 dumps shop buy P2020-012 dumps dumps su cheap P2020-012 dumps cc : paypal : banks : bulk cc . | dumps, sell, good, best, track2, dump, fresh, cvv2, paypal, rescator, carders, market, online, check, approved, cheap, swiped, swiped1, cards, buybest
dumpsportal.com | dumpsportal it certification exam portal | best it certification braindumps portal on web. you can download any exam of your choice without pay any additional charges. dumpsportal.com has over 100 top vendors including microsoft, oracle, cisco, hp, ibm, comptia and many more. | dumps, portal, latest, best, download, braindump, dumpsportal, questions
passiteasy.com | passiteasy it certification exam portal | best it certification braindumps portal on web. you can download any exam of your choice without pay any additional charges. passiteasy.com has over 100 top vendors including microsoft, oracle, cisco, hp, ibm, comptia and many more. | dumps, portal, latest, best, download, braindump, passiteasy, questions
examcollections.info | latest braindump2go it exam P2020-012 dumps pdf & vce free download | braindump2go collects all hot exam cisco, microsoft, comptia, vmware, oracle, checkpoint, emc, hp, ibm, citrix, juniper, avaya, sap P2020-012 dumps here for free | dumps, exam, braindump2go
dumpswheel.net | welcome to P2020-012 dumps wheel - P2020-012 dumps wheel | clear P2020-012 exam for sure 100 % guaranty. download P2020-012 dumps free practice test valid ceh v9 P2020-012 dumps download |
cardertools.su | cvv & P2020-012 dumps t1/t2 shop | online P2020-012 dumps shop, buy cc :: buy P2020-012 dumps :: sell cc :: sell P2020-012 dumps t1-t2 :: cvv :: P2020-012 dumps :: paypal :: ssn :: dob :: full cc info :: bank logins | sell, good, dumps, fresh, track2, cvv2, dump, carders, paypal, market, check, online, cards, shop, cheap, best
braindumps.net | test brain P2020-012 dumps | perfect certification sample questions | free braindumps exam certification | free certification practice tests dumps, popular linux certification exams, the best ccna study guide, requirements for it certification, actual study tools for it certification! | dumps, braindumps, brain, exam, dump, comptia, cisco, simulator, download, mcse, braindump, microsoft, ccna, oracle
free-braindumps.net | 4,500+ exam dumps: pass your it certification exam with free-braindumps.net | free-braindumps.net carries over 3, 800 it exam braindumps that allow you to pass it exams from the first try guaranteed! real exam questions, simulation, pdf guides. | dumps, braindumps, brain, exam, dump, comptia, cisco, simulator, download, mcse, braindump, microsoft, ccna, oracle
jasonshauling.com | jasons hauling | official website | construction hauling, tractor trailer dumps, tri-axle dumps, tandem dumps | |
mybraindumps.net | reliable it certification exams preparation - latest braindumps at mybraindumps.net | mybraindumps.net is a reliable source for quality it certification exam P2020-012 dumps and preparation materials. they help you pass your it exams hassle-free: guaranteed! | dumps, braindumps, brain, exam, dump, comptia, cisco, simulator, download, mcse, braindump, microsoft, ccna, oracle
omerta.vc | omerta - the world is yours | omerta.cc - carders community, carding forum. buy dumps, buy cc+cvv, cashout dumps, skimmers, real carding. | dumps, cvv2, sell, credit, cards, sale, dump, good, forum, card, security, carding, skimmer, hacked, fake, track2, fraud, shop, carders, underground
omerta.bz | omerta - the world is yours | omerta.cc - carders community, carding forum. buy dumps, buy cc+cvv, cashout dumps, skimmers, real carding. | dumps, cvv2, sell, credit, cards, sale, dump, good, forum, card, security, carding, skimmer, hacked, fake, track2, fraud, shop, carders, underground
omerta.cc | omerta.cc - carders community, carding forum. buy dumps, buy cc+cvv, cashout dumps, skimmers, real carding | omerta.cc - carders community, carding forum. buy dumps, buy cc+cvv, cashout dumps, skimmers, real carding. | dumps, cvv2, sell, credit, cards, sale, dump, good, forum, card, security, carding, skimmer, hacked, fake, track2, fraud, shop, carders, underground
surebraindumps.com | sure braindumps - most updated dump portal for all top it certifications 2016 | 3 months free update with pass guarantee for all it certifications. quality P2020-012 dumps with 24/7 customer support. | dumps, brain, braindumps
darkstuff.name | carding forum - carders forum - professionally managed community | darkstuff.name | darkcarder.net is fully featred carding forum . lot of profassionals carders already joined us. they have P2020-012 dumps with pin, western union transfer, etc. | dumps, sell, cvv2, cards, credit, fraud, good, track2, dump, carding, shop, carders, forum, card, best, check, online, fresh, with, skimmer
testdumps.com | braindumps free study guide & P2020-012 dumps for microsoft exam | all study guide about microsoft certification, lead you pass microsoft certification exam, msce, mcp, mcitp, mcts, mcdba, mcdst, mcpd, windows 7 and so on | microsoft, dump, dumps, exam
mcsdexams.com | testking microsoft mcsd P2020-012 dumps free download | latest 70-480 | 70-483 | 70-484 dumps | |
harcotrucking.com | harco trucking - sparks, nv: heavy haul, transfers, low beds, belly dumps, end P2020-012 dumps and more | |
validdumps.com | download valid P2020-012 dumps certification P2020-012 dumps 2016-pass guaranteed questions and answers | |
dumpshop.cc | dump + pin online shop :: track1 track2 + pin :: buy cc :: buy P2020-012 dumps :: 101 and 201 P2020-012 dumps :: | |
mcsebraindumps.com | braindumps free certification exam | braindumps practice exams | braindumps test questions | microsoft exam cost, vmware questions, isc certification hardware, cisco certification help, it certification training, free it certification exams. | dumps, braindumps, brain, exam, dump, comptia, cisco, simulator, download, mcse, braindump, microsoft, ccna, oracle
vcp550dumps.com | free and latest braindump2go exam questions | all braindump2go latest microsoft, vmware, comptia, cisco,hp ,citrix and some other hot exams practice tests & questions and answers free download! | ciscio new updated: new updated 350-080 exam questions from braindump2go 350-080 pdf P2020-012 dumps and 350-080 vce dumps! welcome to download the newest braindump2go | exam, test, dumps, study, guide, questions, center, book, actual, brainfump, ccie, data, written
crdpro.su | carder.pro - carding forum - ??????? ?????. ????????? ? ???? (carding, card fraud, carding forum, carders forum, carders board, credit card fraud, fraud, atm fraud, atm skimmer, P2020-012 dumps shop, credit cards cvv, credit cards cvv2, dumps, P2020-012 dumps with pin, cvv2, buy dumps, buy credit cards, P2020-012 dumps with pin) - powered by vbulletin | carding, card fraud, carding forum, carders forum, carders board, credit card fraud, fraud, atm fraud, atm skimmer, P2020-012 dumps shop, credit cards cvv, credit cards cvv2, dumps, P2020-012 dumps with pin, cvv2, buy dumps, buy credit cards, buy creditcard, buy cvv, buy cvvs, d+p, sell dumps, buy dumps, buy cvv, buy cvv2, sell dumps, sell track2, buy track2, buy cards, cheap cvv, buy cvv, sell cvv, fresh cvv, good cvv, buy good cvv, sell good cvv, best cvv, check cvv, cvv2 dump, buy cvv online, sell cc, dump shop | dumps, sell, cvv2, cards, credit, fraud, good, track2, dump, carding, shop, carders, forum, card, best, check, online, fresh, with, skimmer
procrd.su | carder.pro - carding forum - ??????? ?????. ????????? ? ???? (carding, card fraud, carding forum, carders forum, carders board, credit card fraud, fraud, atm fraud, atm skimmer, P2020-012 dumps shop, credit cards cvv, credit cards cvv2, dumps, P2020-012 dumps with pin, cvv2, buy dumps, buy credit cards, P2020-012 dumps with pin) - powered by vbulletin | carding, card fraud, carding forum, carders forum, carders board, credit card fraud, fraud, atm fraud, atm skimmer, P2020-012 dumps shop, credit cards cvv, credit cards cvv2, dumps, P2020-012 dumps with pin, cvv2, buy dumps, buy credit cards, buy creditcard, buy cvv, buy cvvs, d+p, sell dumps, buy dumps, buy cvv, buy cvv2, sell dumps, sell track2, buy track2, buy cards, cheap cvv, buy cvv, sell cvv, fresh cvv, good cvv, buy good cvv, sell good cvv, best cvv, check cvv, cvv2 dump, buy cvv online, sell cc, dump shop | dumps, sell, cvv2, cards, credit, fraud, good, track2, dump, carding, shop, carders, forum, card, best, check, online, fresh, with, skimmer
ebonycumdumps.us | ebony cum P2020-012 dumps - ebonycumdumps.com video clips and pics | slutty sistas that beg to swallow cum but it's not always something they want to do. watch ebony cum P2020-012 dumps movies and pictures here. |
pass4suredumps.com | pass4sure dumps | updated pass4sure P2020-012 dumps are available now. real exam question answers study material. |
testkingdumps.com | testking dumps | testking P2020-012 dumps are available at discount price. download braindumps study material. |
sqldumpster.com | sql database resources & data base P2020-012 dumps for mysql, csv, access & oracle | sql resources including sql database tutorials and data base P2020-012 dumps for mobile app developers and website designers. sql database resources & data base P2020-012 dumps for mysql, csv, access & oracle | dumps, database, home
onlyexamdumps.com | latest exam P2020-012 dumps | pass in first attempt | cheap exam dumps | latest exam P2020-012 dumps | pass in first attempt | cheap exam P2020-012 dumps | buy any exam certification P2020-012 dumps in very cheap price | clear any exam certification |
easttexastrailers.org | east texas trailers | single axles utility trailer carhauler gooseneck P2020-012 dumps gooseneck dumps, tilts. | gooseneck, dumps, tilts, carhauler, trailer, axles, utility, single
ebonycumdumps.com | ebony cum P2020-012 dumps - black hoes swallowing white nut | black hoes swallow white man jizz on ebony cum dumps! | ebony, swallowing, mouth, white, cock, play, blowjob, dump, dumps, black, interracial, hood, hoodrat
kentuckianatrucking.com | kentuckiana trucking - hauling earth | for more than 40 years kentuckiana trucking has built a reputation of being a dependable regional logistics company specializing in bulk hauling with end dumps. they strive to always provide economical, safe transportation services which exceed their customers' expectations | logistics, dumps, hauling, dozer, trailer, certified, disposal, flat, trucks, regional, delivery, freight, bulk, dump, tractor
mcsadumps.com | ensurepass microsoft mcsa P2020-012 dumps free download | the P2020-012 dumps from mcsadumps specifically address the microsoft certified solutions associate (mcsa) certifications. |
passforsure.co | passforsure exam P2020-012 dumps with real questions answers | updated passforsure P2020-012 dumps are available now. 100% exam passing and money back guarantee. |
xxxdumps.com | xxx dumps | free amateur gallery - xxx dumps | gallery, porn, teens, babes, dumps, amateurs, amateur, image
dumpspdf.com | dumps pdf real exam question answers from dumpspdf.com | latest and updated real exam P2020-012 dumps pdf available at discount price. |
carderpro.biz | carderpro.biz - private carding forum - (carding, card fraud, carding forum, carders forum, carders board, credit card fraud, fraud, atm fraud, atm skimmer, P2020-012 dumps shop, credit cards cvv, credit cards cvv2, dumps, P2020-012 dumps with pin, cvv2, buy dumps, buy credit cards, P2020-012 dumps with pin) | carding card fraud carding forum carders forum carders board credit card fraud fraud atm fraud atm skimmer P2020-012 dumps shop credit cards cvv credit cards cvv2 P2020-012 dumps dumps with pin cvv2 buy P2020-012 dumps buy credit cards buy creditcard buy cvv buy cvvs d+p sell P2020-012 dumps buy P2020-012 dumps buy cvv buy cvv2 sell P2020-012 dumps sell track2 buy track2 buy cards cheap cvv buy cvv sell cvv fresh cvv good cvv buy good cvv sell good cvv best cvv check cvv cvv2 dump buy cvv online sell cc dump shop | dumps, sell, cvv2, cards, credit, fraud, good, track2, dump, carding, shop, carders, forum, card, best, check, online, fresh, with, skimmer
certifydumps.com | cisco ccna mcse mcts oracle 11i comptia a+ professional it certify dumps | professional it certify P2020-012 dumps available online cisco ccna, microsoft mcse mcts, oracle 10g 11i comptia a+. their certify P2020-012 dumps study questions fastest success in any it certification exams | comptia, oracle, certification, ccsp, ccnp, tools, training, dumps, microsoft, mcts, ccna, cisco, mcse, certify
monetki.cc | ??????? | | sell, dumps, carding, cards, cvv2, good, credit, dump, forum, paypal, fresh, track2, shop, carder, best, online, drop, cvvs, carders, community
crimes.ws | crimes.ws :: elite carding forum :: | crimes.ws - elite carding forum for elite carders & hackers only regarding credit card security, atm security, atm shims, buy track 2, carding forum, carders forum, carders bbs, carders board, underground community, infraud buy dumps, buy skimmer, hacked cc, hack cvv, uk dob, eu dob, buy track2, skimmed dumps, hacked dumps, P2020-012 dumps for sale, sale cvv2, dump fullz, skimmer for sale, fake passport sale, fake driver license, cashout drops, plastic holograms, carder fraud forum, selling dumps, cashout dumps, atm fraud | dumps, sale, forum, carders, skimmer, fake, hacked, cashout, security, fraud, fullz, dump, cvv2, carder, selling, holograms, driver, license, passport, drops
csu.su | csu.su - private carding forum - (carding, card fraud, carding forum, carders forum, carders board, credit card fraud, fraud, atm fraud, atm skimmer, P2020-012 dumps shop, credit cards cvv, credit cards cvv2, dumps, P2020-012 dumps with pin, cvv2, buy dumps, buy credit cards, P2020-012 dumps with pin) | carding card fraud carding forum carders forum carders board credit card fraud fraud atm fraud atm skimmer P2020-012 dumps shop credit cards cvv credit cards cvv2 P2020-012 dumps dumps with pin cvv2 buy P2020-012 dumps buy credit cards buy creditcard buy cvv buy cvvs d+p sell P2020-012 dumps buy P2020-012 dumps buy cvv buy cvv2 sell P2020-012 dumps sell track2 buy track2 buy cards cheap cvv buy cvv sell cvv fresh cvv good cvv buy good cvv sell good cvv best cvv check cvv cvv2 dump buy cvv online sell cc dump shop | dumps, sell, cvv2, cards, credit, fraud, good, track2, dump, carding, shop, carders, forum, card, best, check, online, fresh, with, skimmer
pass4suredumps.net | pass4suredumps.net | prepare for pass any it certification exam success | questions, dumps, test, practice, exam, braindump
vceexams.com | vce exams | vce certification software, download vce dumps | vce exam and testing software, vce test engine and technology behind it, best resource for vce dumps, certification files and vce exam simulator. |
vceplayer.com | vce player | vce dumps, vce test engine | the latest vce player and vce test engine; certification exam dumps, vce file downloads, free vce software, vce braindumps. |
kivibros.com | kivi bros. trucking - duluth, mn - home | kivi bros. trucking of duluth, mn, is an experienced leader in the transportation industry and offers trucking logistics, heavy hauling, step decks, conestogas, flatbed trailers, and hauling with end P2020-012 dumps and belly dumps. they serve the u.s. nationwide and canada. | dumps, hauling, handlers, material, large, canada, construction, step, transfers, conestogas, decks, nationwide, equipment, cloquet, belly, heavy, logistics, flatbed, trailers, transportation
startrailersales.com | star trailer sales, inc. - (800) 324-8167 | star trailer sales, inc. of no. mankato mn is your source for new & used semi trailers; grain hoppers, flats, drops, livestock, vans, reefers, end dumps, side dumps, tag trailers and more. | trailers, stock, trailer, dumps, sales, felling, cornhusker, benson, fontaine, barrett, transcraft, paul, mike, jeff, jake, doug, timpte, stoughton, wilson, drop
updateddumps.com | updated P2020-012 dumps | get all latest and updated exam dumps, like no where!! | |
myresearchinstitute.com | braindumps, notes, books for free, dumps, submit your braindump | they provide free braindumps, books, notes, and help you achive your targets | dumps, solaris, brain, developer, linux, mcse, master, ccie, mcsd, java, upgrade, scsa, certified, programmer, associate, cissp, designer, administrator, cwna, scjp
pass4suredumps.in | pass4sure P2020-012 dumps india | pass4sure india site for their indian customers. now you can purchase in indian rupees and download P2020-012 dumps at discount. |
danpalmertrucking.com | dan palmer trucking home | | county, beds, dumps, flat, rock, shop, truck, boxes, anderson, shasta, tehama, transfers, lassen, trinity, trucks, siskiyou, modoc
pass4king.com | pass4king - they provide all kinds of it certification exam study guidance and brain dumps | pass4king gives the candidates the latest update version microsoft, cisco, oracle and comptia certification training and study materials. their high quality brain P2020-012 dumps and q&as are the most accurate study guide material about the it certification exam. they provide their customers 100% pass guarantee. | exam, certification, material, redhat, microsoft, dumps, cisco, comptia, vmware
ccbase.biz | ccbase.biz /trusted/ buy cvv2 shop, track2 P2020-012 dumps shop, sell cvv2, buy dumps, buy track2, cvv2.ch fulls online shop | buy cvv2, cvv2 shop, track2 shop, P2020-012 dumps shop, sell paypal, buy paypal, fresh cvv2, sell, buy dumps, buy track2, buy cvv, sell dumps, sell track2, buy track2, buy cards, cheap cvv, buy cvv, sell cvv, fresh cvv, good cvv, buy good cvv, sell good cvv, best cvv, carding, market, check cvv, cvv2 dump, buy cvv online | sell, track2, cvv2, good, dumps, shop, fresh, paypal, check, dump, market, online, cards, cheap, best, carding
rustandsons.com | rust & sons trucking | san diego, ca | family owned & operated since 1976 | rust & sons trucking in el cajon, california operates refrigerated and dry van trailers, and has an aggregate division for dirt and materials. specializing in end dumps, hazardous waste, super dumps, 10-wheelers, flatbeds, water tanks, and roll-offs. |
istqbdumps.blogspot.in | istqb P2020-012 dumps 2015 with answers | istqb question P2020-012 dumps 2015 | istqb sample paper 2015 with answer | |
certs4you.com | certs4you real exam dumps | get real exam P2020-012 dumps with 100% passing guarantee. certs4you real exam questions answers pdf and test engine software. |
latestdumps.net | updated braindumps 2016 for all top certifications | latestdumps.net | latestdumps.net is rated among the best dump providers in the certification market. pass guarantee, free updates, money back guarantee with 24/7 customer support. | exam, braindumps, dumps, questions
exams4sure.net | exams4sure exams question answers dumps | get exams questions answers P2020-012 dumps with 100% passing guarantee. exams4sure real exam questions & answers pdf and test engine. |
freepass4sure.com | free pass4sure | get free pass4sure P2020-012 dumps pdf. download and pass your exam. | test, questions, answers, practice, pass4sure, download, dumps, free
gotocert.com | get your it certification easily. gotocert expert team is ready to help you. | best it certification real exam practice material provider. exam P2020-012 dumps for cisco, comptia, oracle, ibm, sun and more. | exam, practice, dumps, real, materials, certification
certleader.com | certleader - leader of it certifications, latest dumps, guaranteed pass | lead to pass your certification exams quickly and easily, try their practice test and study guide free today! | questions, answers, study, guide, exam, test, dumps, practice, certleader
do4exam.com | do4exam- professional it certification exam P2020-012 dumps provider. they do all things to help with your exams. | they do all things to help with your exams. get prepared for all your it certification exams with do4exam latest study materials. | dumps, brain, exams, brainitdump, certification
ccnapass4sure.com | ccna pass4sure exam questions dumps: ccnapass4sure | get ccna pass4sure braindumps and pass your exam easily. real exam questions dumps |
sy0-301dumps.com | sy0-401 P2020-012 dumps | pass4sure comptia security+ sy0-401 exam questions & answers practice test dumps | pass4sure comptia security+ sy0-401 exam questions & answers practice test dumps |
sidedump.com | side dump smithco | side dump trailers | smithco side dump | side dump trailers - smithco side dump trailers are #1 in number of side dump models offered, #1 in number of side dump trailers produced, #1 in number of side dump trailers sold, #1 in resale value in the industry, and #1 in payload capacity. | side, dump, dumps, smithco, trailers, belly, volumen, dumping, information, industry, trailer, manufacturers, high
aaatrailersales.net | home | aaa trailer sales | flatbed, equipment, utility, and fuel tank trailers for sale in petty, tx sand blasting,trailer repairs,custom welding | aaa trailer sales sells singe axle utility trailers, car haulers, gooseneck car haulers, deck over goosenecks, dumps, gooseneck dumps, tilt trailers, tanks, welding trailers and they also do sand blasting, trailer repairs, custom welding | tank, water, trailer, dumps, petty, trailers, utility, sand, welding, custom, repairs, blasting, dealer, diesel, goosenecks, gooseneck, haulers, traps, singe, tanks
hanaexamdumps.com | home - hana exam dumps | sap hana exam dumps |
cheaptry.com | cheaptry: best it certification material provider are cheapest in the market! | cheaptry are cheapest in the market and provides exam brain P2020-012 dumps for all the latest and most popular it certification exams.such as cisco, microsoft, comptia, vmware, ibm, oracle, citrix and so on. | exam, practice, dumps, test, study, certification, guide
testkings.com | testking - it certification exams - original questions and answers - success guaranteed | testking - true updated real exam questions. success in mcitp, mcts, mcdst, mcdba, ccna, ccnp, ccie, a+, network+, security+ and many more. | certification, questions, answers, test, exam, dumps, question, real, brain, online, pool, training, practice, document, braindumps, study, documents, original, questionsdumps, braindump
braindumpgalaxy.com | latest exam P2020-012 dumps questions and answers in pdf format | their exam dump helps you pass your certification exams at first try. | format, answers, exam, dumps, questions, latest
examfull.com | examfull the most professional it certification exam preparation guide. help you to pass all your certification exams easily. | get your microsoft, cisco, comptia, oracle, ibm, sun, juniper, hp and all it certifications easily with the help of examfull here you can find all latest it certification exam study guide. try examfull free demo first. | dumps, practice, certification, latest, exam
blackhatcorp.com | black hat corporation - best of carding | black hat corporation - best of carding. join the best carding forum community today. | cardable, carding, dumps, credit, card, website, game, review, fraud, 2016, dump, forum, guide, best, corporation, hacking, black, checker, shop
cvvshop.net | cvv shop net :: cc shop : paypal shop : P2020-012 dumps shop : cvv shop net | cvv shop net :: cc shop : paypal shop : P2020-012 dumps shop : cvv shop net | shop, online, code, card, sell, store, credit, website, seller, russia, good, admin
californiamaterials.com | california materials, inc. - aggregate & trucking, stockton, ca | located in stockton, california materials inc. is a service provider to the aggregate and trucking industry, mobile rock crushing and materials | dumps, double, yard, bottom, recycle, super, transfers, hauling, aggregates, trucking, mobile, crushing, rock, stockton
ajgtrucking.ca | untitled document | ajg trucking company, drivers in edmonton, ajg, truck drivers in edmonton, tandem trucks, edmonton fastest truck, best drivers in edmonton, end dumps, tri-end dumps, quads, quad wagon, super bee trucks and trailers | edmonton, truck, services, fastest, trucks, trucking, drivers
dumpscerebrais.com.br | dumps cerebrais | |
examdumpsportal.com | dumps portal | |
cams-dumps.blogspot.com | cams dumps | |
fe-dumps.su | fe-dumps.su | login | |
itbibles.com | latest cisco, microsoft, ibm, hp, oracle, comptia, citrix, vmware exam dumps. get your certification easily- itbbibles | itbibles is the leading provider of it certification exam practice questions and answers. they have microsoft, cisco, comptia, oracle, ibm, sun, juniper, hp and all popular certification exam q&as. 100% money back guarantee. | exam, practice, certification, test, dumps, provider, cert, material
fundumps.com | like, share & enjoy - fun dumps | |
freedumps.net | home - free dumps | |
windycitydumps.com | windy city dumps | |
wildmountaindesigns.com | wild mountain designs | wild mountain designs | tees, designs, wild, printing, screen, shirts, dumps, mountain, corporate, wholesale, vancouver, hockey, retro, buddah, going, funny, sale, awesome, canada, gold
examreal.com | examreal tests | get success in passing your certification exam at first attempt | it certification test, they at examreal provide 6000+ certification exam P2020-012 dumps & study guide. pass at first attempt with examreal! | exam, examreal, certification, practice, dumps, test, provider, real, material, cert
19thcenturybottlediggers.com | privy's and P2020-012 dumps - 19th century bottle diggers | bottles dug from privy s & dumps. the dig story s in my blogs were published in ab&gc, western & eastern treasure and bottles & extras magazines. | digging, bottles, tops, bottle, antique, privy, flasks, trash, hobby, finding, historical, bitters, houses, pits, quack, glass, outhouse, collecting, cork, dump
cehv9ccnadumpsdownload.net | dumps download - download certified ethical hacker version 9 ceh v9 P2020-012 dumps free download. ownload free practice tests for such certifications as mcse, mcsa, mcsd, a+, network+, security+, ccie, ccna, ccnp, and so on | download certified ethical hacker version 9 ceh v9 P2020-012 dumps free download. ownload free practice tests for such certifications as mcse, mcsa, mcsd, a+, network+, security+, ccie, ccna, ccnp, and so on |
centralvalleyconcrete.com | cvc & cvt main | the san joaquins premier ready mix and trucking provider with over 30 years of experience. | valley, concrete, central, demolition, bottom, trucking, dumps, hauling, class, rock, services, fill, sand, base, dirt, trucks, redi, ready, rocks, aggregate
cisexams.com | free cisco exams learning center - pass4sure questions answers P2020-012 dumps free for all | free cisco certification exam questions answers for your practice test. prepare pass4sure practice test. | exam, questions, testking, pass4sure, cisco, dumps, certifications, free
zapili.cc | dumps cvv scans marketplace - index page | |
vcp5-dcv.com | vcp5-dcv P2020-012 dumps vmware exam material | |
smo.nu | smonu | programming, ramblings, and brain dumps. |
ccvfree.blogspot.com | free credit/debit card dumps | |
4dexcavating.net | full-service excavating company odessa texas | oil field construction services and equipment, demolition services and equipment, bulldozers, haul trucks, excavators, backhoes, end dumps, water trucks, rollers and belly P2020-012 dumps texas - 4d excavating | contact their full-service excavating company today at (432) 940-9715 for all your oil field construction services and equipment, demolition services and |
octavian.su | octavian.su - buy P2020-012 dumps shop & credit cards with cvv2 | |
lampeduza.net | rescator.cm - buy P2020-012 dumps shop & credit cards with cvv2 | |
bravos.ir | rescator.cm - buy P2020-012 dumps shop & credit cards with cvv2 | |
pmiexam.net | top pmi exam | pmi questions answers practice test dumps | |
cardershop.su | cardershop :: buy cc :: sell cc :: cvv :: P2020-012 dumps :: paypal :: ssn :: dob :: full cc info :: | |
pass4surenow.com | pass4sure P2020-012 dumps at pass4surenow | pass it exams easily | |
blacklotus.ws | blacklotus.ws | ccv good and fresh, P2020-012 dumps track 1/2, bank login, ccv 2, visa, maestro, fullz info, random cc, disco cc, amex credit card, bin, dob, cc with dob, cc wiht bin, visa cc, master cc, amex cc, discoveri gold card, master standart cards, platinum cards, business card, debit card, tracks 1&2, hsbc bank login, barclays bank login, welsfargo bank login, boa account hacked, chase bank logins, credit union username and password, halifax login, free credit cards, paypal dumps, ccv list, ccv dumps, track 1, track 2, cc with pin, database dump, free cc, account dump, payza accounts | login, bank, card, cards, credit, dumps, track, account, master, free, with, amex, dump, visa, union, username, logins, accounts, hacked, chase
freshbuddies.com | freshbuddies: verbal reasoning questions and answers, general knowledge questions and answers, networking questions and answers, database questions and answers,interview questions and answers, ccna questions and answers , aptitude questions and answers for bank cat mat tancet competitive exams, general aptitude questions and answers for tnpsc rrb exams, general english questions and answers, oracle questions and answers, linux interview questions and answers, ccna interview questions and answers, windows certification questions and answers, linux certification questions and answers, non verbal reasonig questions and answers, oracle questions and answers, db2 questions and answers, mysql questions and answers, sql server questions and answers, c programming questions and answers, c++ programming questions and answers, java programming questions and answers, windows server questions and answers - freshbuddies | general knowledge questions and answers, networking questions and answers for tests, interview and all competitive exams, database interview questions and answers, active directory , dns, sharepoint interview questions, ccna, ccnp, general english, general science, general knowlege, verbal reasoning, non verbal reasoning, gk for competitive exam, ccnp routing, ccnp switching, ccna dump questions, ccnp dump questions, oracle questions, oracle interview questions, oracle dump questions, db2 interview questions, db2 exam questions, windows networking, sharepoint server interview questions, mysql interview questions, mysql dumps, c programming questions, c++ programming questions, java programming questions, window server 2003 , windows server 2008 interview questions, windows server 2003 interview questions, windows server 2003 dumps, windows server 2008 dumps | questions, interview, server, windows, freshbuddies, dumps, networking, general, 2003, ccnp, answers, 2008, reasoning, database, programming, dump, aptitude, verbal, oracle, routing
validservice.cc | valid service | sell cvv , cc P2020-012 dumps , cvv , paypal , wu and bank transfers . | |
cvvzone.su | cvvzone.su - best cvv and P2020-012 dumps services bulk offers availble enjoy | |
xbib.org | linked open data P2020-012 dumps | jrg prante - software developer | |
accesswellnessgroup.com | counseling & employee assistance program in lexington ky, access wellness group | access wellness group provides a variety of counseling & employee assistance programs in the lexington ky area and throughout central kentucky. 2014 latest 117-102 P2020-012 dumps 070-294 P2020-012 dumps replica omega for man ysl replica handbag for sale best prada replica handbags | access, wellness, group, lexington, employee, assistance, program, counseling
braindumpsdeals.com | brain-dumps deals | prepare with actual questions for 100% results in exam | |
70-410dumps.com | 70-410 P2020-012 dumps - microsoft exam preparation notes | question answers test | |
jagtrucking.com | jag trucking inc. - trucking company, aggregate delivery, tri axle dump trucks | jag trucking inc. trucking company, aggregate delivery, tri axle dump trucks, end dumps, belly dumps |
foxstories.info | girlfriend abruptly P2020-012 dumps boyfriend, asks for him back following unprecendented jackpot win | fox stories | |
exam2pdf.com | exam2pdf | exam2pdf, certifications it vce to pdf, dumps | dumps, certifications, exam2pdf
carder.pw | carding forum - ??????? ????? - carding site | carding forum. credit cards, carder, dumps, hacking, cvv, cc, stuff carding, enroll, vendor, free cvv, skimmer, skimming. ??????? ?????, ??????, ?????, ????????? ????? | free, hacking, dumps, carder, cards, stuff, credit, vendor, enroll, carding
mazuri-rmd.blogspot.com | random mind dumps | a blog to list, link, or discuss my latest romance and erotic stories. |
phenomsworld.com | phenom's world | a peek into the mind of a wallflower | living, brain, dumps, family, finding, cancer
infofanz.com | education and information network | education and information about student projects, dissertation guide, it certification dumps, student assignments, top universities, best colleges | student, assignments, universities, best, colleges, dumps, certification, projects, dissertation, guide, education
wegottrailers.com | affordable trailers and truck accessories, utility trailers, dumps, lowboys, horse, car haulers, rentals, in san antonio, texas, big tex trailers, cm trailers, cargo, new braunfels, texas, san antonio | your one stop shop for trailer sales and service along with the leading truck accessories for the road! complete line of rental and custom built trailers installation services include truck and trailer wiring, tag-a-long hitches, gooseneck hitches, and more. two locations in the san antonio area to better serve you | trailers, texas, antonio, cargo, rentals, utility, accessories, truck, dumps, lowboys, affordable, horse, haulers
fuckingdumpit.com | free adult videos | free adult link dump | free xxx porn videos | free adult link dump to submit porn videos and xxx sex movies | porn, link, adult, free, video, dumps, dump, linkdump, pics, links, videos, directory, clips, gallery, linkdumps, thumbnail, post, posts, fuckingmotherfucker
29auto.net | 29 auto outlet * commercial truck sales * warrenton, va * dump trucks * box trucks * cargo vans * 4x4 * snow plow trucks | 29 auto outlet, warrenton virginia serves maryland, pennsylvania, west virginia, north carolina, delaware, new jersey, new york with commercial truck sales. they stock a wide selection of dump trucks equipped with landscape dump bodies, contractor dumps, grain body dumps, mason dumps, hydraulic and electric hoist. they also stock stake body, concrete body, flat beds, cargo vans, utility bodies. they have trucks for all contractors, masons, electricians | truck, commerical, service, leasing, f350, f450, sales, supplier, magnum, 3500, 2500, 4500, 5500, f550, 6500, ohio, f750, rating, weight, tommy
feshop-store.cc | feshop-store.cc :: buy cc :: sell cc :: cvv :: P2020-012 dumps :: paypal :: ssn :: dob :: full cc info :: bank logins :: tracks :: carder tools :: cvv shop site :: cardershop | |
ecbw.ca | early canadian bottle works | milks, sodas, ginger beers, primitives, crocks and more! | bottles, bottle, canadian, canada, soda, digging, ginger, pontil, antique, dumps, beers, squat, pontilled, iron, brampton, medicines, paper, label, open, hutchinson
pinkshop.name | www.pinkshop.name / buy cvv2, buy cvv, buy track2, buy P2020-012 dumps shop sell | selle | sell
vmwarehelpcenter.com | vmware help center - real questions answers P2020-012 dumps for vmware exams | |
sfdcworld.com | sfdc world - all in one place for salesforce - one place for all salesforce admin/developer tutorial, certification dumps, interview question, ebooks download, job search, real time tasks, discussion forum and more. | one place for all salesforce admin/developer tutorial, certification dumps, interview question, ebooks download, job search, real time tasks, discussion forum and more. |
tego.biz | tego.biz|??????? ?????|carding forum, ?????? credit cards ? dumps,dating,?????? ????? ?????????,dating,skam,?????? ?????? | ??????? ?????, ?????? ????? ?????????, ??????, credit cards, ?????? ? ?????????, ?????, dumps, dating, skam, , ??????, ?????? ??????, ?????? ? ?????????, ?????? ??????? ?????, ?????? ??????? ??, ?????? ????? | skam, cards, credit, dumps, dating
oraclecertified.com | oracle certified: oracle P2020-012 dumps questions answers | |
zzwglobal.com | zzw global, inc. | zzw global is a privately held company and industry leader for oil and gas field services including roustabouts and rig washing to soil farming and end dumps. |
thepokeymansproject.tumblr.com | the pokeymans project | pokemon drawn from description alone by someone who doesnt know anything about pokemon. by noelle stevenson aka gingerhaze submit a pokeyman! | like, truck, dumps, what, thighs, submission
highplainsdrilling.com | high plains drilling | idaho, utah, wyoming, hawaii, irrigation wells, domestic wells, monitoring wells, municipal wells for cities, subdivisions, churches, gravel, dirt hauling, sales, basements, trenches, trackhoe, canal and ditch work, backhoe, dumptruck, belly dumps | wells, canal, trenches, ditch, trackhoe, backhoe, dumps, belly, dumptruck, basements, work, hauling, municipal, monitoring, domestic, cities, subdivisions, irrigation, dirt, gravel
malloystruckequipment.com | home | weld install fabricate repair truck equipment body utility landscape stake lift gate hitch ladder rack plow spreader flat bed small dumps |
carding.bz | carding forum - carding - credit cards - P2020-012 dumps - tracks - laptops shipping - bank accounts - western union transfer | carding forum, carding, carders, western union transfer, illegal credit cards, credit card, cc, tracks, dumps, pin, dell alienware, hacking, botnet, security, paypal, bank account, login, porn xxx, passwords, email, leads, liberty reserve, ukash, webmoney, shipping laptops, cheap electronics, bank transfer | bank, credit, transfer, carding, passwords, email, account, porn, leads, login, reserve, cheap, electronics, laptops, shipping, ukash, webmoney, liberty, botnet, illegal
atlanticstartrailers.com | atlantic star trailers | atlantic star trailers is your full line dealership, specializing in new and used semi-trailer sales, rentals, service, and parts. their semi trailers include tanks, flatbeds, dumps, vans, reefers, lowbeds and most specialized equipment. atlantic star trailers prides itself in representing the industrys leading manufacturers, which include wabash-fruehauf, lbt, clement, mac, talbert, landoll, chevron, transcraft, and more. | clement, talbert, transcraft, chevron, landoll, lowbeds, reefers, service, rentals, sales, parts, tanks, vans, dumps, flatbeds
coinodeal.com | carding forum - carders - credit cards - P2020-012 dumps - tracks - laptops shipping - bank accounts - western union transfer | coinodeal.com - elite carding forums for elite carders & hackers only regarding carding forum, carding, carders, western union transfer, illegal credit cards, credit card, cc, tracks, dumps, pin, dell alienware, hacking, botnet, security, paypal, bank account, login, passwords, email, leads, liberty reserve, ukash, webmoney, shipping laptops, cheap electronics, bank transfer, coino | forum, bank, carding, transfer, credit, hacking, passwords, leads, email, coino, account, login, liberty, reserve, electronics, laptops, shipping, webmoney, ukash, cheap
unixorder.com | carding forum - carders - credit cards - P2020-012 dumps - tracks - laptops shipping - bank accounts - western union transfer | welcome to unixorder.com! elite carding forums for elite carders & hackers only regarding carding forum, carding, carders, western union transfer, illegal credit cards, credit card, cc, tracks, dumps, pin, dell alienware, hacking, botnet, security, paypal, bank account, login, passwords, email, leads, liberty reserve, ukash, webmoney, shipping laptops, cheap electronics, bank transfer, unix | forum, bank, carding, transfer, credit, hacking, passwords, leads, email, unix, account, login, liberty, reserve, electronics, laptops, shipping, webmoney, ukash, cheap
darkmills.cc | dark millions - biggest carding forum - cvv - fullz - P2020-012 dumps - track 1 and 2 - bank logins - bank transfers - western union transfers - hacked bitcoins for sale - legit sellers | dark millions - carding forum - cvv - P2020-012 dumps - fullz - bank logins - hacked credit cards - hacked bank accounts - bank transfers - western union transfers - legit carding forum - legit hacker - legit seller - legit - hacking forum - carder forum - darknet forum - dark web carding forum - tor market - tor forum - private carding forum - how to hack tutorials | forum, carding, legit, bank, transfers, hacked, dark, darknet, carder, private, hack, tutorials, hacking, market, western, fullz, dumps, millions, logins, credit
dump4exam.com | 2016 braindumps,100% guaranteed | best it certification braindumps.we including microsoft, oracle, cisco, hp, ibm, comptia and many more real exclusive certification dumps. |
gbxemu.com | gbxemu | #1 source for gameboy emulators | .nds and .3ds files dot nds in the most popular extension for nintendo ds rom files. other alternatives include .bin for binary P2020-012 dumps of the rom | emulator, emulators
cca175certification.com | cca 175 certification - cloudera spark and hadoop developer exam | become a certified hadoop developer. require cca 175 spark and hadoop certification or cloudera hadoop developer certification to reach your goal. get P2020-012 dumps and exam material. | certification, hadoop, developer, cloudera, exam, dumps, spark
webbone.net | webbone.net :: carding forum - carders - credit cards - P2020-012 dumps - tracks - laptops shipping - bank accounts - western union transfer | webbone.net - elite carding forums for elite carders & hackers only regarding carding forum, carding, carders, western union transfer, illegal credit cards, credit card, cc, tracks, dumps, pin, dell alienware, hacking, botnet, security, paypal, bank account, login, passwords, email, leads, liberty reserve, ukash, webmoney, shipping laptops, cheap electronics, bank transfer, bitcoin, webbone | forum, bank, transfer, carding, hacking, credit, liberty, leads, passwords, account, login, reserve, email, shipping, bitcoin, webbone, electronics, cheap, webmoney, laptops
mcsdquestion.com | braindumps microsoft mcsd P2020-012 dumps free download | mcsdquestion.com is your it certification test prep and training leader. prepare for your microsoft certification exam . download free exams dumps! | exam, questions, real
truckandtrailerworld.com | truck and trailer world in baton rouge, la | dealership of enclosed cargo, goosneck and utility trailers for sale load trail pj trailers arising industries equipment P2020-012 dumps tilts car haulers and golf carts |
coder1.com | mike milano | articles, code snippets, and brain P2020-012 dumps by mike milano | |
mastercvv.cm | mastercvv: login | mastercvv is a underground marketplace auto shop for carders, fresh cvv, dumps, track 1 and track 2, bank account, paypal account and etc... lowest price on best validate 'no log' |
trailer101.com | 101 trailer & rv | | | mendocino, county, haulers, landscapes, equipment, tiltbeds, enclosed, dumps, utility, lake, vehicles, recreational, willits, ukiah, trailer, trailers
thebakingauntie.com | the baking auntie | homemade, baked, goods, making, homemade, goodies, for, all, your, occasions, | gooey, goods, homemade, pecan, pecans, goodie, good, candies, cakes, candy, dump, dumps, dumped, cake
westerntruck.net | western truck & trailer | construction trailers, smithco, ranco, load king, talbert, saw mfg, cps/manac, retesa, advance engineered products bottom dumps, side dumps, end dump, lowboys, pneumatics, guppy/pigs, tilt deck, water tanks, frac tanks | dump, truck, decks, deck, western, drop, trailer, finance, step, sales, tilt, pups, belly, lowboy, side, lowboys, tandem, dollies, haul, dolly
passcometrue.com | passcometrue - pass all it certification exams and make your dream come true | try passcometrue free demo of cisco, comptia, hp, ibm, microsoft exams and so on. | dumps, brain, exams, brainitdump, certification
pass4it.com | pass4it - your reliable partner and professional it certification exam material provider. | most comprehensive it exam materials help you pass all your it certification exams easily. | dumps, brain, exams, brainitdump, certification
ineed2pass.com | ineed2pass - pass all it certification exams easily with their real exam practice. | try ineed2pass free demo of cisco, comptia, hp, ibm, microsoft exams and so on. | dumps, brain, exams, brainitdump, certification
brain2pass.com | brain2pass - your professional it certifications expert. provide training for any exam! | brain2pass.com guarantee their candidates get their certification at their first try. they also have money back guarantee to assure of the pass. | material, provider, training, dumps, exams, brain, brain2pass
dumpstree.com | try dumpstree free demo of cisco, comptia, hp, ibm, microsoft exams and so on. | dumpstree - pass all it certification exams easily with their real exam practice. latest update and experts revised. | dumps, brain, exams, brainitdump, certification
vceplayer.net | vce player | vce test engine, vce software | vce software, vce dumps, test engine, vce certification exam download, vce test preparation, and more to get certified with vce. |
reliancetrailer.com | reliance trailer - reliance trailer home | reliance trailer designs and manufactures the most complete line of vocational trailers offered by any supplier. from chip haulers to transfer dumps, reliance offers a trailer specifically engineered for your applications. |
sierrarentalandtransport.com | sierra rental and transport - heavy haul, reno, sparks, northern nevada, northern - home | | trucking, heavy, companies, hauling, equipment, cargo, haul, trucks, water, freight, movers, services, dumps, machinery, best, flatbeds, loading, interstate, transformation, extraction
cooldump.com | cool dump - the most cool viral video dumps | cool dump is your ultimate source for the most cool viral videos. their mission is to make your life happier. |
jstrailers.com | j.s. enterprises | jeff and sandy suchon's trailer sales located west of crivitz, wisconsin off highway w. retailer of continental cargo trailers including cargo trialers, auto carriers, race car trailers, e-z dumpers, and more. all styles are available including utility, dumps, enclosed, and goosenecks. |
sparkstrailers.com | sparks trailers - flatbed trailer | sparks trailers- headquarters for: flatbed trailers, dumps, cargo trailers, stock trailers & mowers. with the following brands: pj, eby, american hauler, & badboy! |
flydumps.com | flydumps expert team is will help you to get all it certifications easily. | all hottest it exam certification real questions and answers. flydumps is the most reliable provider of it certification exams study material. surely help you pass your exams successfully. | dumps, brain, exams, brainitdump, certification
pass4king.net | pass4king latest it certification exam braindumps vce download | free download pass4king testking pass4sure it exam P2020-012 dumps vce files. 100% guaranteed latest updated real exam questions and answers, 99.8% pass rate. |
northernatlanticfinancial.com | northern atlantic financial - tree & landscape equipment financing | providing financing for arborists, landscapers & tree services nationwide. they finance chip trucks, bucket trucks, landscape trucks, dumps, stump grinders & more. |
bildmix.com | bildmix.com - tglich neue picdumps! | bildmix.com - wir prsentieren dir tglich neue, lustige und sexy picdumps aus den unterschiedlichsten bereichen! | bilder, witzig, komisch, lustig, dumps, picdumps
ccnproute.net | offer new updated microsoft exam P2020-012 dumps with pdf & vce | free offer latest 70-341, 70-342, 70-331, 70-332, 70-457, 70-458, 70-243 , 70-246, 70-247 exam questions | exam, questions
passin1day.com | pass your exam in 1 day with passin1day.com | get latest P2020-012 dumps from passin1day and pass your exam in first attempt |
itcertbibles.com | itcertbibles it certification practice materials. the most professional and accurate real exam q&as. | itcertbibles real it exam questions and answers: vmware, ibm, hp, oracle, citrix, cisco, microsoft, comptia and so on. | exam, dump, dumps, real, demo
tsoftit.com | tsoft it - free online learning center in bangladesh | tsoft it, which is organized by some helping young talent. its a modern information technology learning center. this organization wants to help people who have | ccna, tutorial, mikrotik, exam, training, bangla, video, answers, bangladesh, book, academy, full, configuration, router, free, list, dumps, questions, course, study
donleytrucking.com | donley trucking - williamsville, illinois | illinois trucking service. 3rd generation trucking company, centrally located just outside of springfield illinois. | illinois, midwest, deliveries, dumps, over, local, road, products, delivery, trucking, construction, demo, quarry, delivered
examsec.com | examsec is the most reliable it exam material provider. they provide the most accurate | examsec is a leading provider of it certification materials: latest real it certification exam questions and answers materials. | dumps, brain, exams, brainitdump, certification
theuglyindian.com | the ugly indian | the ugly indian, theuglyindian, theuglyindian, it's the 'system', stupid, it's their corrupt governments, oh, it's the uneducated people, because they are all ugly indians | theuglyindian, litter, cigarette, death, traps, urination, public, dumps, stains, indian, ugly, filth, paan, footpath, open
pjontario.com | pj trailers on - home | established in 2008, pj trailers ontario inc. is a family owned business, proud to be an exclusive dealer of pj trailers. they are developing a reputation of having some of the best pricing of pj trailers in the ontario market place. they pride ourselves on customer service, and will always strive to exceed their customers expectations. | trailers, ontario, dumps, utilities, flatdecks, tilts, carhaulers
careercert.info | careercert.info build your career with it certifications | free study guides, practice exams and training materials for it certifications. | workbook, video, actualtest, testinside, exam, pass4sure, book, study, demand, class, bootcamp, guide, test, braindumps, ccdp, ccda, ccie, ccnp, ccna, ccvp
king4exam.com | king4exam - find all popular it exam certification study materials here. their expert team is ready to help you to get your certification easily. | king4exam is the most proffessional and reliable it training material and prictice material provider. 100% pass rate and money back guarantee policy. try their free demo to check if that is the right one you want to buy. | exam, practice, certification, dumps, king4exam
examsbibles.com | examsbibles - help all candidates pass the it certification exams easily. | accurate it exam materials: vmware, ibm, hp, oracle, citrix, cisco, microsoft, comptia and so on. help you to pass the exam quickly. | exam, practice, certification, test, dumps, provider, cert, material
cmdtrailers.com | truck and trailer sales, semis, live floors, dumps, new, used, jacksonville, miami, fl, ga, al tn, sc | new and used commercial truck and trailers for sale. they have dump trailers, live floor trailers, semi trucks and more. they are in jacksonville fl with worldwide sales and shipping. stop by their jacksonville lot or view their online inventory. |
itdumpscool.com | itdumpscool - help all candidates pass the it certification exams easily. | itdumpscool helps you with all your it certification exams. 100% real exam questions and answersl update according to the latest real exam. | dumps, brain, exams, brainitdump, certification
examcheap.com | examcheap - help all candidates pass the it certification exams easily. | examcheap helps you with all your it certification exams. 100% real exam questions and answersl update according to the latest real exam. | dumps, brain, exams, brainitdump, certification
linzer-tattooatelier.at | kunst, die unter die haut geht - linzer tattooatelier | in gelassener atmosphre am rande der obersterreichischen landeshauptstadt, fern von stress und alltag werden individuelle dot-, geometric- und watercolortattoos mit viel liebe zum detail und mit allerhchster akribie verewigt. | dumps, julia, urfahr, schmidinger, tattooartist, linz, lederfabrik, watercolor, tattoo, tattooatelier, dots, geometric, aquarell, geometrie, linzer
real4pass.com | as a leading it exam study guides provider, real4pass provides the latest real test practice for hottest cisco, microsoft, comptia, vmware, ibm, hp, oracle, citrix exams. 100% real and latest. | real4pass - the most professional provider of all it certifications. pass all the it exam easily | dumps, brain, exams, brainitdump, certification
certbus.com | certbus tests | get success in passing your certification exam at first attempt | it certification test, they at certbus provides 7000+ certification exam P2020-012 dumps and study guide. pass at first attempt with certbus! | exam, certification, dump, brain
examcrowd.com | examcrowd - latest update source for all it certification exams. | cisco, comptia, oracle, ibm, sun and more, leading provider of the latest and best exam practice for the it certification exams. | certification, practice, exam, study, dumps, guides
hijackerhydraulics.com | hijacker hydraulics | hijacker hydraulics - finest hydraulic product line! | hydraulic, hijacker, pumps, dumps, italian, motors, hopping, switches, cups, springs, pumpheads, projacker, suspension, finest, hydraulics, equipped, exclusive, econojacker, dealer, marzocchi
bucknertrailers.com | bucknertrailers | bucknertrailers is located in western north carolina and offers a well built utitliy trailer at a reasonable price. they carry most sizes and can order cusom trailers, enclosed trailers, goosenecks and dumps. | trailers, custom, order, dump, goosenecks, enclosed, utility
letsmovesomething.com | home :: santella's shufffle | santella's shuffle, dave santella, david santella, 804-234-1515, 804-234-1525, trucking, trucks, semi trucks, loads, freight, truckload, less than truckload, ltl, dispatching, carriers, shippers, brokers, 3pl, third party logistics, freight, dry van, flatbed, stepdeck, reefer, refrigerated, expedited, tanker, 53' containers, rail, intermodal, shipping, united states, canada, north america, end dumps, owner operator, dump trailers, trucking webs | freight, truckload, trucks, trucking, santella, reefer, intermodal, shipping, united, refrigerated, containers, tanker, states, expedited, rail, america, operator, dump, trailers, webs
topvce.com | topvce - help candidates on all it certification exams preparation. pass it certification exams, get it certifications easily. | topvce provides latest real test questions and answers for all it certifications. you are guaranteed with their best quality. | exam, dump, dumps, real, demo
examboom.com | examboom - help you to pass all it certification exams! | pass your cisco, microsoft, comptia, vmware, ibm, hp, oracle, citrix and other exams! get certified easily! examboom guarantees your success in all your certification exams. 100% pass rate or you'll get your money back. | exam, answers, dumps, questions, exams, certification
jumpexam.com | jumpexam- leading provider on all it certification real exam practice and test questions and answers. | jumpexam it exam real questions and answers. guarantee the pass of corresponding it exams - microsoft, cisco, ibm, sun, juniper, hp, a+, ccna, mcitp, comptia, oracle and all others. | exams, dumps, brain, jumpexam
testbells.com | testbells - certification with money back assurance | get the latest certification exams with 100% passing guarantee at testbells.com. pass any exam in your first attempt or get your money back. | exams, testking, dumps, pass4sure, testbells, certs, test, bells, certification, tests
itcertstore.com | itcertstore - 100% real it certification exam questions and answers. easily pass with a high score. | itcertstore helps you to prepare for all it certification exams. 100% latest update according to the latest real exam. | exam, dump, dumps, real, demo
freshexam.com | freshexam - 100% real it certification exam questions and answers. easily pass with a high score. | freshexam helps you pass all it certification exams. 100% real and update according to the latest real exam. | dumps, brain, exams, brainitdump, certification
gallery-dump.eu | gallery-dump.eu - daily dose of porn gallery dumps | gallery dump - browse millions of free porn photos in thousands of porn galleries. amateur porn photos and ultimate hand picked babe porn photos collection along with user submitted galleries. |
theeverydaycarry.com | everyday carry | everyday carry, or edc are items people carry everyday. everyday carry gear reviews, edc bag P2020-012 dumps and best edc items for you! | wallets, tools, knives, flashlights, dump, carry, gear, everyday, tactical
itcertbible.com | itcertbible - pass all it certification exams easily with their real exam practice. latest update and experts revised. | first-hand it exam questions and answers help you pass the exam in first time. try itcertbible free demo of cisco, comptia, hp, ibm, microsoft exams and so on. | exam, dump, dumps, real, demo
dumps4download.us | pass4sure free cisco exam questiosns and answers| free online quiz | study without P2020-012 dumps is very tough here with the help of dumps4download website you can get free pass4sure cisco exam questions and answers free of cost. online quiz system to check your preparation. |
testking.com.pk | testking offers all it certification P2020-012 dumps bundle in $99 | in bundle offer all exams questions and answers of microsoft, cisco, oracle, ibm, hp, vmware, comptia and many more are included. six months free updates. | free, testking, test, exam, online, exams, practice, king, microsoft, certification, oracle, ccna, mcse
geneticvisions.net | genetic visions, inc., tests dna from cattle to identify genes influencing production traits, hair color and animal health and viability. alterations in the composition of dna provide "genetic markers". a genetic marker associated with a trait of interest can be used in cattle breeding programs as a selection tool. | genetic visions, inc., tests dna from cattle to identify genes influencing production traits, hair color and animal health and viability. genetic visions, inc. advances genomic testing capabilities through new equipment investment and accelerated genetics incorporates genomic predictions into sire selection program. | marker, genetic, genomics, markers, bovine, testing, test, blad, dumps, coat, reproductive, genomic, color, martin, calpain, free, genetics, freemartin, advantages, sire
passapply.com | passapply - 100% real it certification exam questions and answers. easily pass with a high score. | passapply helps you to prepare for all it certification exams. 100% latest update according to the latest real exam. try the free demo first. | exam, dump, dumps, real, demo
lazyresources.tumblr.com | snorlax's lazy resources | where a snorlax ran P2020-012 dumps all the resources a snorlax ran wants. | icons, animal
super4test.com | super4test - the most professional provider of all it certifications. pass all the it exam easily. | super4test best exam practice help you pass the exam in one shot. free demo of cisco, comptia, hp, ibm, microsoft exams and so on. | exam, practice, certification, test, dumps, provider, cert, material
victorygardensinc.com | victory gardens mulch - supplier of mulch, topsoil, stones and more in southeastern pa | one of the largest manufacturers and suppliers of bulk mulch in montgomery, bucks, chester and delaware counties. their family owned business has been producing mulch from yard debris and other organic materials since 1990. they sell bulk mulches both wholesale and retail and deliver products with their own fleet of trailers, tri-axle P2020-012 dumps trucks and six wheeler retail trucks. | mulch, county, stone, planting, firewood, topsoil, screened, dirt, montgomery, retail, trucks, chester, bucks, playground, materials, delaware, dyed, bulk, sparks, britton
me-equip.com | modern electronics & equipment | manufacturer of food processing equipment specalizing in pecan processing | pecan, continuous, shelling, crackers, sanitizers, dumps, barrel, elevators, roasters, dryer, blowers, bins, sizers, processors, nuts, equipment, shellers, processing, sorters, inspection
vcebox.com | vcebox - 100% real it certification exam questions and answers. easily pass with a high score. | vcebox helps you to prepare for all it certification exams. 100% latest update according to the latest real exam. try the free demo first. | exam, practice, certification, test, dumps, provider, cert, material
itexamcup.com | itexamcup - pass all it certification exams easily with their real exam practice. latest update and experts revised. | first-hand it exam questions and answers help you pass the exam in first time. try itexamcup free demo of cisco, comptia, hp, ibm, microsoft exams and so on. | exam, practice, certification, test, dumps, provider, cert, material
itexam911.com | itexam911 - provide the latest it real exam practice questions and answers. | pass all it certification exams easily with itexam911 real exam practice. try free demo to trail the quality and accuracy of xxx real exam questions and answers. | dumps, brain, exams, brainitdump, certification
certificationkey.com | certificationkey real exam question answers dumps, pass4sure braindumps | certification training with guaranteed preparation. exam practice questions and answers, pass4sure braindumps. | exam, test, preparation, download, actualtests, braindumps, question, answers, pass4sure

RSS Killexams P2020-012 dumps

CNN

Fox News

Google News




Article 1 | Article 2 | Article 3 | Article 4 | Article 5 | Article 6 | Article 7 | Article 8 | Article 9 | Article 10 |
Back to Exam List


Exam dumps Books list